Temporal phase evolution OCT for measurement of tissue deformation in the human retina in-vivo
Open Access
- 25 October 2021
- journal article
- research article
- Published by Optica Publishing Group in Biomedical Optics Express
- Vol. 12 (11), 7092-7112
- https://doi.org/10.1364/boe.440893
Abstract
We demonstrate the use of temporal phase evolution (TPE-) OCT methods to evaluate retinal tissue deformation in-vivo over time periods of several seconds. A custom built spectral domain (SD)-OCT system with an integrated retinal tracker, ensuring stable imaging with sub-speckle precision, was used for imaging. TPE-OCT measures and images phase differences between an initial reference B-scan and each of the subsequent B-scans of the evaluated temporal sequence. In order to demonstrate the precision and repeatability of the measurements, retinal nerve fiber (RNF) tissue deformations induced by retinal vessels pulsating with the heartbeat were analyzed in several healthy subjects. We show TPE maps (M-scans of phase evolution as a function of position along B-scan trace vs. time) of wrapped phase data and corresponding deformation maps in selected regions of the RNF layer (RNFL) over the course of several cardiac cycles. A reproducible phase pattern is seen at each heartbeat cycle for all imaged volunteers. RNF tissue deformations near arteries and veins up to similar to 1.6 mu m were obtained with an average precision for a single pixel of about 30 nm. Differences of motion induced by arteries and veins are also investigated.Keywords
Funding Information
- Austrian Science Fund (P30378)
This publication has 63 references indexed in Scilit:
- Motion artifact and speckle noise reduction in polarization sensitive optical coherence tomography by retinal trackingBiomedical Optics Express, 2013
- Real-time eye motion correction in phase-resolved OCT angiography with tracking SLOBiomedical Optics Express, 2012
- Strain estimation in phase-sensitive optical coherence elastographyBiomedical Optics Express, 2012
- Phase-sensitive imaging of the outer retina using optical coherence tomography and adaptive opticsBiomedical Optics Express, 2011
- Real-time bulk-motion-correction free Doppler variance optical coherence tomography for choroidal capillary vasculature imagingOptics Express, 2011
- Depth profiling of photothermal compound concentrations using phase sensitive optical coherence tomographyJournal of Biomedical Optics, 2011
- The Relationship between Retinal Microvascular Abnormalities and Coronary Heart Disease: A ReviewThe American Journal of Medicine, 2010
- Phase-sensitive optical coherence tomography imaging of the tissue motion within the organ of Corti at a subnanometer scale: a preliminary studyJournal of Biomedical Optics, 2010
- Retinal vascular image analysis as a potential screening tool for cerebrovascular disease: a rationale based on homology between cerebral and retinal microvasculaturesJournal of Anatomy, 2005
- Recovery of images from atmospherically degraded short-exposure photographsThe Astrophysical Journal, 1974