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Comparative Study between Western Blotting and Automatic Quantitative Analysis on Protein

雷 虹
Published: 1 January 2021
Hans Journal of Biomedicine , Volume 11, pp 113-119; doi:10.12677/hjbm.2021.112015

Abstract: 目的:比较传统的蛋白免疫印迹和全自动蛋白质定量分析检测特异性蛋白质相对含量的差异,并进行方法学优化,为蛋白质定量分析提供技术参考。方法:分别使用两种系统对不同分子量的目标蛋白进行检测,比较两种系统的灵敏度、重复性和适用范围。结果:相比传统WB系统,全自动系统灵敏性、重复性和稳定性更高,并且可进行多分子同时检测,尤其在检测微量蛋白、分子量较大或较小的蛋白以及高通量方面具有优势。结论:全自动蛋白检测是一种灵敏、高效的检测特异性蛋白丰度变化的系统,值得在科研和医学检验中推广。 Objective: To compare the difference between traditional Western Blotting (WB) and automatic quantitative protein analysis on specific protein relative content , and to optimize the methodology, so as to provide technical reference for protein quantitative analysis. Methods: In order to compare the sensitivity, repeatability and applicable range of the two methods. WB and automatic quantita-tive protein analysis are used to detect the target molecules, which with different molecular weights. Results: Compared with traditional WB, WES had higher sensitivity, repeatability and stability, and can carry out multi-molecule detection, which has high throughput especially in the detection of trace proteins or proteins with larger or smaller molecular weights. Conclusion: WES is a sensitive and efficient method to detect the changes of abundance of specific protein, which is worth popular-izing in scientific research and medical examination.

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