Survey of plant growth promoting and antagonistic traits in winter wheat grain endophytic bacteria
Open Access
- 30 July 2021
- journal article
- Published by OU Scientific Route in EUREKA: Life Sciences
- No. 4,p. 66-72
- https://doi.org/10.21303/2504-5695.2021.001978
Abstract
The aim of this work was to isolate endophytic bacteria from wheat grains and to evaluate their plant growth promoting traits (PGPT) as well as an inhibitory effect on P. syringae pv. atrofaciens (McCulloch) growth. Endophytic bacteria were isolated by a culture-dependent protocol from the grains of winter wheat variety of Ukrainian selection Podolyanka with high resistance to syringae. Totally 2.7±0.09 CFU/1 g of dry wheat grain were isolated, ten cultivable bacterial isolates were obtained. Spore-forming bacilli predominated in the wheat grain endophytic community. Gram-negative fermenting and non-fermenting rod-shaped bacteria and Gram-positive cocci were also present. Seven out of ten isolates possessed numerous plant growth promoting traits including phosphate solubilization, oligonitrotrophy, and indolic compound producing. Two isolates possessed antagoniscic activity against syringae in vitro along with plant growth promoting features. According to biochemical profiling and mass-spectrophotometric identification, these two isolates were assigned to Paenibacillus and Brevibacillus genera. These endophytic bacteria can be considered as promising objects for agrobiotechnology. However, more research is needed to confirm their biotechnological potential in planta experimentsKeywords
This publication has 29 references indexed in Scilit:
- Assessing wheat production futures in the UkraineOutlook on Agriculture, 2016
- Disease Impact on Wheat Yield Potential and Prospects of Genetic ControlAnnual Review of Phytopathology, 2016
- Optimization of isolation and cultivation of bacterial endophytes through addition of plant extract to nutrient mediaMicrobial Biotechnology, 2015
- Endophytic bacteria from wheat grain as biocontrol agents of Fusarium graminearum and deoxynivalenol production in wheatMycotoxin Research, 2015
- In vitro antagonistic activity, plant growth promoting traits and phylogenetic affiliation of rhizobacteria associated with wild plants grown in arid soilFrontiers in Microbiology, 2014
- Characterisation of endophyticBacillus thuringiensisstrains isolated from wheat plants as biocontrol agents against wheat flag smutBiocontrol Science and Technology, 2014
- Multicenter Evaluation of the Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Gram-Positive Aerobic BacteriaJournal of Clinical Microbiology, 2013
- A Review of the Studies and Interactions ofPseudomonas syringaePathovars on WheatInternational Journal of Agronomy, 2012
- Genetic Diversity and Phylogeny of Antagonistic Bacteria against Phytophthora nicotianae Isolated from Tobacco RhizosphereInternational Journal of Molecular Sciences, 2011
- Phosphate solubilization activity of rhizobia native to Iranian soilsPlant and Soil, 2006