Dipotassium Glycyrrhizininate Improves Skin Wound Healing by Modulating Inflammatory Process
Open Access
- 14 February 2023
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 24 (4), 3839
- https://doi.org/10.3390/ijms24043839
Abstract
Wound healing is characterized by a systemic and complex process of cellular and molecular activities. Dipotassium Glycyrrhizinate (DPG), a side product derived from glycyrrhizic acid, has several biological effects, such as being antiallergic, antioxidant, antibacterial, antiviral, gastroprotective, antitumoral, and anti-inflammatory. This study aimed to evaluate the anti-inflammatory effect of topical DPG on the healing of cutaneous wounds by secondary intention in an in vivo experimental model. Twenty-four male Wistar rats were used in the experiment, and were randomly divided into six groups of four. Circular excisions were performed and topically treated for 14 days after wound induction. Macroscopic and histopathological analyses were performed. Gene expression was evaluated by real-time qPCR. Our results showed that treatment with DPG caused a decrease in the inflammatory exudate as well as an absence of active hyperemia. Increases in granulation tissue, tissue reepithelization, and total collagen were also observed. Furthermore, DPG treatment reduced the expression of pro-inflammatory cytokines (Tnf-α, Cox-2, Il-8, Irak-2, Nf-kB, and Il-1) while increasing the expression of Il-10, demonstrating anti-inflammatory effects across all three treatment periods. Based on our results, we conclude that DPG attenuates the inflammatory process by promoting skin wound healing through the modulation of distinct mechanisms and signaling pathways, including anti-inflammatory ones. This involves modulation of the expression of pro- and anti-inflammatory cytokine expression; promotion of new granulation tissue; angiogenesis; and tissue re-epithelialization, all of which contribute to tissue remodeling.Funding Information
- CNPq (305402/2019-6, 303837/2018-7)
- Coordination of Superior Level Staff Improvement (CAPES) scholarship
This publication has 133 references indexed in Scilit:
- Dipotassium Glycyrrhizate Inhibits HMGB1-Dependent Inflammation and Ameliorates Colitis in MicePLOS ONE, 2013
- Influence of interleukin-8 (IL-8) and IL-8 receptors on the migration of human keratinocytes, the role of PLC-γ and potential clinical implicationsExperimental and Therapeutic Medicine, 2011
- Phenotypic and functional plasticity of cells of innate immunity: macrophages, mast cells and neutrophilsNature Immunology, 2011
- NF-κB in the regulation of epithelial homeostasis and inflammationCell Research, 2010
- Role of prostaglandins in fibroblast activation and fibrosisJournal of Cell Communication and Signaling, 2010
- A mutation in Irak2c identifies IRAK-2 as a central component of the TLR regulatory network of wild-derived miceThe Journal of Experimental Medicine, 2009
- The Role of Vascular Endothelial Growth Factor in Wound HealingJournal of Surgical Research, 2009
- Interleukin-1 Receptor-associated Kinase 2 Is Critical for Lipopolysaccharide-mediated Post-transcriptional ControlOnline Journal of Public Health Informatics, 2009
- The IL-8 Protease SpyCEP/ScpC of Group A Streptococcus Promotes Resistance to Neutrophil KillingCell Host & Microbe, 2008
- The involvement of the interleukin-1 receptor-associated kinases (IRAKs) in cellular signaling networks controlling inflammationCytokine, 2008