Nanometer materials and the repairing function of ligament injury in basketball

Abstract

Basketball has always been a favorite sport for many young people, but ligament injuries often occur during basketball, which will also affect the progress of many sports games. This study focuses on the effects of nanomaterials on the repair of basketball ligament injuries, mainly through tissue engineering to repair ligaments. First, HA nanocomposites are prepared by dissolving P (LLA-Cl) and collagen in hexagonal fluoropropanol. Samples were scanned and analyzed using a high-resolution micro-CT system. Scanning electron microscope (SEM) was used to examine the microstructure, chemical structure and thermal stability of the composite biomaterial. The ligament maturity is evaluated by measuring ligament growth (Tb.N). In vitro cell culture technique was used to inoculate bone marrow mesenchymal stem cells on the cultured nanomaterials, and the adhesion and proliferation of cells were observed with confocal microscope and SEM. The ability of bone marrow mesenchymal stem cells to secrete extracellular calcium matrix is determined by alcohol phosphatase (ALP) and calcium ion content. Using RT-PCR and immunohistology, bone formation and ligament differentiation of bone marrow mesenchymal stem cells were detected. Nanomaterials show extremely high compatibility with damaged ligament tissue, Lysholm score increased from 43.2 ± 2.4 points (30–88 points) before surgery to 96 ± 2.0 points (72–100 points) after surgery (p < 0.001). The research results show that nanocomposite materials combined with tissue engineering can obviously promote the repair of ligaments.