Evaluation of influence of IL-6 C-572G gene polymorphism and clinical factors on positive platelet antibody test
Open Access
- 15 January 2021
- journal article
- Published by Heighten Science Publications Corporation in Journal of Advanced Pediatrics and Child Health
- Vol. 4 (1), 006-012
- https://doi.org/10.29328/journal.japch.1001023
Abstract
Background: Interleukin-6 (IL-6) promotes antibody production. The objective of this study was to investigate whether IL-6 C-572G single nucleotide polymorphisms (SNP) and clinical factors are associated with positive platelet antibody test. Materials and methods: Thirty platelet recipients with platelet antibodies (responders) and 20 platelet recipients without platelet antibodies (non-responders) were randomly selected. The -572 C>G (rs 1800796) SNPs in the promoter region of IL-6 gene were genotyped by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method. Solid phase red cell adherence assay (SPRCA) was used for platelet antibody detection. Results: Age, sex, percentage patients with benign diseases, and percentage of patients with homozygotes for the C allele at position -572 of the IL-6 gene were similar between responders and non-responders. Although the amounts of platelets pheresis transfused to patients with hematologic diseases were higher than those of non-hematologic diseases (47.2 ± 54.2 vs. 17.4 ± 13.8 units, p = 0.019), detection rate of platelet antibodies was lower in patients with hematologic diseases than that in patients with non-hematologic diseases (42.3% vs. 79.2%, p = 0.01). Conclusion: There was no association between IL-6 C-572G gene polymorphism and positive reactivity in solid phase platelet antibody detection method in platelet recipients.Keywords
This publication has 31 references indexed in Scilit:
- Screening plateletpheresis donors for HLA antibodies on two high-throughput platforms and correlation with recipient outcomeTransfusion, 2010
- The influence of class II transactivator and interleukin‐6 polymorphisms on the production of antibodies to donor human leucocyte antigen mismatches in renal allograft recipientsInternational Journal of Immunogenetics, 2009
- Regulatory T-cell status in red cell alloimmunized responder and nonresponder miceBlood, 2009
- The induction of antibody production by IL-6 is indirectly mediated by IL-21 produced by CD4+ T cellsThe Journal of Experimental Medicine, 2009
- Reciprocal developmental pathways for the generation of pathogenic effector TH17 and regulatory T cellsNature, 2006
- Universal prestorage leukoreduction in Canada decreases platelet alloimmunization and refractorinessBlood, 2004
- Absence of anti‐D alloimmunization in hematologic patients after D‐incompatible platelet transfusionsTransfusion, 2002
- The effect of novel polymorphisms in the interleukin-6 (IL-6) gene on IL-6 transcription and plasma IL-6 levels, and an association with systemic-onset juvenile chronic arthritis.JCI Insight, 1998
- INDUCTION OF TH1 AND TH2 CD4+T CELL RESPONSES:The Alternative ApproachesAnnual Review of Immunology, 1997
- Biological and clinical aspects of interleukin 6Immunology Today, 1990