Potential Antibacterial Activity of Bioactive β-sitosterol from Root Bark of Rhizophora apiculata from Lampung Coastal
Open Access
- 27 March 2021
- journal article
- Published by Institute of Research and Community Services Diponegoro University (LPPM UNDIP) in Jurnal Kimia Sains dan Aplikasi
- Vol. 24 (4), 114-119
- https://doi.org/10.14710/jksa.24.4.114-119
Abstract
β-sitosterol is an essential bioactive phytosterol naturally present in plant cell membranes. It has a coincident structure with animal cholesterol. This investigation reported isolation, structure analysis, and an antimicrobial assay of β-sitosterol from the root bark of Bakau Minyak (Rhizophora apiculata) from Lampung coastal. The isolation of β-sitosterol was carried out through maceration using methanol, separation by vacuum liquid chromatography (VLC), and purification by column chromatography (CC) using ethyl acetate/n-hexane (2:8) as eluent. The structure of β-sitosterol was determined using spectroscopic analysis (UV-Vis, FT-IR, 13C-NMR, 1H-NMR, DEPT, and GC-MS). The pure β-sitosterol has 107.4 mg of white needle crystalline compound, the compound melting point about 140.7-141.2oC, the molecular mass confirmed by m/z 414, and UV absorption detected at λ 203.9 nm. The β-sitosterol antimicrobial bioactivity assay has shown potential activity to be developed as a lead compound against E. coli.Keywords
This publication has 21 references indexed in Scilit:
- An update on β-sitosterol: A potential herbal nutraceutical for diabetic managementBiomedicine & Pharmacotherapy, 2020
- Beta-sitosterol attenuates insulin resistance in adipose tissue via IRS-1/Akt mediated insulin signaling in high fat diet and sucrose induced type-2 diabetic ratsEuropean Journal of Pharmacology, 2020
- Secondary metabolites from mangrove plants and their biological activitiesPublished by Elsevier BV ,2020
- Effects of β-Sitosterol from Corn Silk on TGF-β1-Induced Epithelial–Mesenchymal Transition in Lung Alveolar Epithelial CellsJournal of Agricultural and Food Chemistry, 2019
- Effect of β-sitosterol self-microemulsion and β-sitosterol ester with linoleic acid on lipid-lowering in hyperlipidemic miceLipids in Health and Disease, 2019
- In silicoandin vivoanalysis to identify the antidiabetic activity of beta sitosterol in adipose tissue of high fat diet and sucrose induced type-2 diabetic experimental ratsToxicology Mechanisms and Methods, 2019
- Effects of Stigmasterol and β-Sitosterol on Nonalcoholic Fatty Liver Disease in a Mouse Model: A Lipidomic AnalysisJournal of Agricultural and Food Chemistry, 2018
- EVALUATION OF THE ANTI-INFLAMMATORY CAPACITY OF BETA-SITOSTEROL IN RODENT ASSAYSAfrican Journal of Traditional, Complementary and Alternative Medicines, 2016
- Structure elucidation of β-sitosterol with antibacterial activity from the root bark of Malva parvifloraSpringerPlus, 2016
- Immunomodulatory properties of Beta-sitosterol in pig immune responsesInternational Immunopharmacology, 2012