Meta-analysis of rumination behavior and its relationship with milk and milk fat production, rumen pH, and total-tract digestibility in lactating dairy cows

Abstract

Time spent ruminating is affected by diet and affects the rumen environment. The objective of the current study was to conduct a meta-regression to characterize the variation in rumination time and its relationship with milk and milk fat yields and variables mechanis-tically associated with milk fat synthesis, including rumen pH and total-tract digestibility. The analysis included 130 journal articles published between 1986 and 2018 that reported 479 treatment means from lac-tating Holsteins cows during established lactation. Milk yield averaged 34.3 kg/d (range 14.2-52.1 kg/d), milk fat averaged 3.47% (range 2.20-4.60%), and rumen pH averaged 6.1 (range 5.3-7.0). Rumination observation systems were categorized into 6 groups, but there was little difference in average rumination time among sys-tems. The total time spent ruminating averaged 444 min/d (range 151-638 d) and occurred in 13.8 bouts/d (range 7.8-17.4 bouts/d) that averaged 32.7 min (range 20.0-48.1 min). Bivariate regressions were modeled to include the random effect of study, and correlations were evaluated through the partial R-2 that excluded variation accounted for by the random effect. Rumina-tion time was quadratically increased with increasing milk fat yield (partial R-2 = 0.27) and milk fat percent (partial R-2 = 0.17). Rumination was also increased with increasing milk yield, dry matter intake, and rumen pH, and was quadratically related to dietary neutral detergent fiber (NDF) and total-tract NDF digestibility (partial R2 = 0.10-0.27). Similar relationships were ob-served for rumination per unit of dry matter and NDF intake. The best-fit multivariate model predicting total rumination time included milk yield, milk fat yield, and concentration and accounted for 37% of the variation. Total-tract digestibility was available for 217 treatment means; when included in the model, the partial R-2 in-creased to 0.41. Last, principal component analysis was conducted to explore the relationship among variables. The first 2 principal components in the broad analyses explained 36.7% of the 39 variables evaluated, which included rumination bouts and time spent ruminating. In conclusion, rumination time was related to milk fat across a large number of studies, although it explained only a limited amount of the variation in milk fat.