Perfecting Targeting in CRISPR
- 23 November 2021
- journal article
- review article
- Published by Annual Reviews in Annual Review of Genetics
- Vol. 55 (1), 453-477
- https://doi.org/10.1146/annurev-genet-071719-030438
Abstract
CRISPR-based genome editing holds promise for genome engineering and other applications in diverse organisms. Defining and improving the genome-wide and transcriptome-wide specificities of these editing tools are essential for realizing their full potential in basic research and biomedical therapeutics. This review provides an overview of CRISPR-based DNA- and RNA-editing technologies, methods to quantify their specificities, and key solutions to reduce off-target effects for research and improve therapeutic applications. Expected final online publication date for the Annual Review of Genetics, Volume 55 is November 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.Keywords
This publication has 150 references indexed in Scilit:
- High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificityNature Biotechnology, 2013
- CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineeringNature Biotechnology, 2013
- High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cellsNature Biotechnology, 2013
- One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome EngineeringCell, 2013
- Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene ExpressionCell, 2013
- RNA-Guided Human Genome Engineering via Cas9Science, 2013
- Multiplex Genome Engineering Using CRISPR/Cas SystemsScience, 2013
- A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial ImmunityScience, 2012
- An unbiased genome-wide analysis of zinc-finger nuclease specificityNature Biotechnology, 2011
- Crystal structure of the anti-viral APOBEC3G catalytic domain and functional implicationsNature, 2008