Rubber Seed Kernel as Potent Solid Substrate for the Production of Lipase by Pseudomonas aeruginosa Strain BUP2
Open Access
- 1 January 2015
- journal article
- research article
- Published by Scientific Research Publishing, Inc. in Advances in Enzyme Research
- Vol. 03 (02), 31-38
- https://doi.org/10.4236/aer.2015.32004
Abstract
This study explored the utility of flours of rubber seed, coconut and groundnut kernels, and de-oiled cakes of coconut and groundnut as solid substrate for the production of lipase by Pseudomonas aeruginosa strain BUP2 (MTCC No. 5924), a novel bacterium reported from the rumen of Malabari goat. Various proportions (10%, 20%, 30%, 40% or 50%) of flours or cakes were prepared (w/v) with BUP medium (pH 4, 5, 6, 7 or 8), and incubated at different temperature (25°C, 28°C, 30°C or 32°C) for 24 to 96 h. The samples were assayed for lipase activity at 24 h intervals. The rubber seed flour (20%)-BUP medium supported the maximum lipase production (871 U/gds) at 48h incubation (pH 6, 28°C), followed by ground nut flour (398 U/gds), while ground nut cake supported the least lipase production (244 U/gds). From this, it is evident that the cheaply available rubber seed is an efficient substrate for the production of lipase, irrespective of its known demerit that it contains the limarin, a toxin; in fact, we could not detect limarin in the fermented matter. Thus, the utility of rubber seed for the production of a costly enzyme is reported from a novel rumen bacterium, which would be advantageous for rubber farmers.Keywords
This publication has 15 references indexed in Scilit:
- Potato flour mediated solid-state fermentation for the enhanced production of Bacillus thuringiensis-toxinJournal of Bioscience and Bioengineering, 2013
- Production of protease and lipase by solvent tolerant Pseudomonas aeruginosa PseA in solid-state fermentation using Jatropha curcas seed cake as substrateBioresource Technology, 2008
- Response surface method to optimize the production and characterization of lipase from Penicillium verrucosum in solid-state fermentationBioprocess and Biosystems Engineering, 2007
- Oil cakes and their biotechnological applications – A reviewBioresource Technology, 2007
- Green gram husk—an inexpensive substrate for alkaline protease production by Bacillus sp. in solid-state fermentationBioresource Technology, 2006
- Determination of key enzyme activities in commercial peptidase and lipase preparations from microbial or animal sourcesEnzyme and Microbial Technology, 2002
- Solid state fermentation for the synthesis of inulinase from Staphylococcus sp. and Kluyveromyces marxianusProcess Biochemistry, 1999
- Mixed-solid substrate fermentation. A novel process for enhanced lipase production byCandida rugosaActa Biotechnologica, 1998
- Coconut cake – a potent substrate for the production of lipase by Candida rugosa in solid‐state fermentationActa Biotechnologica, 1997
- Optimization of liquid media for lipase production by Candida rugosaBioresource Technology, 1996