Gerbera is used as a cut flower and has gained popularity as ornamental flower and great demand in the world market for ornamental plants. Micropropagation is used to meet the demand for commercial planting material. The objective was to evaluate the BAP and ANA phytoregulators effect gerberas in vitro multiplication. The explants were inoculated in MS culture medium containing different concentrations of BAP (0.0; 2.22; 4.44; 8.88 and 17.76 µmol L-1) and ANA (0.0; 1.34; 2.68 and 5.36 µmol L-1). After eight weeks, the number of shoots formed in each explant and the average length of the shoots were evaluated. Was possible to establish and recommend an ideal concentration of BAP and ANA for each gerbera genotype.