Quantitative evaluation of signaling events in Drosophila S2 cells
- 1 December 2008
- journal article
- Published by Springer Science and Business Media LLC in Biological Procedures Online
- Vol. 10 (1), 20-28
- https://doi.org/10.1251/bpo139
Abstract
Drosophila activates a robust defense response to gram-negative bacteria through the Immune deficiency (Imd) pathway. Imd signaling proceeds through c-Jun N-terminal Kinase (JNK), NF-kB and caspase modules. The individual signaling modules act in a highly coordinated manner to yield a stereotypical response to infection. While considerable attention has focused on NF-kB-mediated antimicrobial activities, more recent studies have highlighted the involvement of JNK signaling in the Imd pathway response. JNK signaling occurs in a transitory burst and drives the expression of a number of gene products through the AP-1 transcription factor. In this report, we describe a simple method for the quantification of JNK activation by Western blot analysis or directly in tissue culture plates.Keywords
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