Kainate-Induced Degeneration of Hippocampal Neurons. Protective Effect of Activation of the Endocannabinoid System
- 23 July 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Bulletin of Experimental Biology and Medicine
- Vol. 171 (3), 327-332
- https://doi.org/10.1007/s10517-021-05221-4
Abstract
We studied the prolonged action of kainic acid on glutamatergic neurons in the dorsal hippocampus and the endocannabinoid-dependent protection against neurodegeneration. The pyramidal neurons of the CA3 field of the hippocampus, as well as granular and mossy cells of the dentate gyrus were examined. Light and electron microscopy revealed substantial damage to the components of the protein-synthesizing (rough endoplasmic reticulum, Golgi apparatus, and polyribosomes) and catabolic (lysosomes, autophagosomes, multivesicular structures, and lipofuscin formations) systems in all cells. Pyramidal and mossy neurons die mainly by the necrotic pathway. The death of granular cells occurred through both apoptosis and necrosis. The most vulnerable cells are mossy neurons located in the hilus. Activation of the endocannabinoid system induced by intracerebral injection of URB597, an inhibitor of degradation of endocannabinoid anandamide, protected the normal structure of the hippocampus and prevented neuronal damage and death induced by KA.Keywords
This publication has 14 references indexed in Scilit:
- The Role of Ubiquitin-Proteasome Pathway and Autophagy-Lysosome Pathway in Cerebral IschemiaOxidative Medicine and Cellular Longevity, 2020
- Advances in understanding hilar mossy cells of the dentate gyrusCell and tissue research, 2017
- Cannabinoids as hippocampal network administratorsNeuropharmacology, 2017
- Neural stem cells and epilepsy: functional roles and disease-in-a-dish modelsCell and tissue research, 2017
- Oxidative Stress Associated with Neuronal Apoptosis in Experimental Models of EpilepsyOxidative Medicine and Cellular Longevity, 2014
- The Developmental Stage of Dentate Granule Cells Dictates Their Contribution to Seizure-Induced PlasticityJournal of Neuroscience, 2010
- Neuronal pigmented autophagic vacuoles: lipofuscin, neuromelanin, and ceroid as macroautophagic responses during aging and diseaseJournal of Neurochemistry, 2008
- Cannabinoids and NeuroprotectionPublished by Springer Science and Business Media LLC ,2008
- Endocannabinoid Enhancement Protects against Kainic Acid-Induced Seizures and Associated Brain DamageThe Journal of pharmacology and experimental therapeutics, 2007
- The Endocannabinoid System Controls Key Epileptogenic Circuits in the HippocampusNeuron, 2006