This study was designed to determine the microbial and heavy metal contamination level in expeller’s groundnut oil. Ten samples of groundnut oil were collected, labeled from E1 to E10 and the microbial and heavy metals examination tests were laboratory assessed. The study results showed that, the Total Count of Bacteria (TCB) for all oil Expellers samples was 33.8 Colony Forming Unit (Cfu)/ml oil. The highestt value was (111 Cfu/ml) in E10 and the lowest value (7 Cfu/ml oil) in E1. Concerning the Total Count of Fungal (TCF) for all samples was 31.4 and 6.75 Cfu/1ml for the yeast and mould respectively. Yeasts highest count was 109 Cfu/ml in E10 and the lowest count was 6 Cfu/ml in E1. while moulds highest count was 18 Cfu/ml in E10 and the lowest number of 2 Cfu/ml oil was found in E2. The bacterial detection test of Staph, Bacillus, Coliform, and E.Coli in the oils Expellers explored that no bacterial presence in E1, E3, and E7 but, the four types were recorded in E5, E9 and E10. On the other hand E2, E6 and E8 showed positive detection to Staph and Bacillus, while E4 was positive to Bacillus detection only. The Concentration of Iron (Fe) on expeller oil samples (E2, E3, E4, E6, E7, E8, E9 and E10) was higher than the minimum specification limit of 5meq/kg for heavy metals, but E1 and E5 were within the minimum specification limit. All expellers oil samples were higher than acceptable limit for Copper (Cu) concentration that established by SSMO and Codex alimentary for heavy metals of less than 0.1meq/kg. As well as, the Concentration value of Lead (Pb) all expeller oil samples were above the specification limit of heavy metals that should not be more than 0.1meq/kg.