The little cashew from the Cerrado (Anacardium humile) is a kind of family anacardiáceaceas, a shrubby plant of great incidence in the Brazilian Cerrado. It is a small pseudofruit, of acidic and juicy flavor, which is known to have colors ranging from yellow to red. It can be consumed naturally or in juices, drinks, sweets. However, its true fruit is the chestnut. Seed germination is influenced by factors such as the substrate, which can be improved in order to improve germination, resulting in the acquisition of more vigorous seedlings. The experiment was conducted in a greenhouse at the Federal Institute of Education, Science and Technology of Tocantins, city of Gurupi/TO, from 26 October 2017 to 15 February 2018. Seeds were collected directly from the pseudofruits. The highest root length value was obtained for seedlings of the following substrates: bs + ws + pb (7.1 cm and 12.5 cm) and ws (6.7 cm and 10.7 cm), intermediate values: or + rs + vl (6.3 cm and 10.0 cm) and hw + vl + sawdust + cp + ws (5.5 cm and 9.5 cm), and lower values on substrates: bs (5.7 cm and 8.0 cm) and bs + rs + sawdust (6,6 cm e 9,0 cm). It was also observed that the highest value referring to the first emergency count and seedling emergence once again stood out the substrates bs + ws + pb (61.0 % and 87.5 %) and ws (56.2% and 86.2 %). Intermediate results were obtained from hw substrates + vl + sawdust + cp + ws(44.0 % and 81.0 %) and or + rs + vl (48.4 % and 81.2 %) and lower for bs substrates (37.5% and 72.0 %) and bs + rs + sawdust (45.3% and 77.0 %). The substrates: bs + ws + pb and washed sand provided the highest values of viability and vigor in cashew seeds.