Expression Profiling of Mitogen-Activated Protein Kinase Genes Reveals Their Evolutionary and Functional Diversity in Different Rubber Tree (Hevea brasiliensis) Cultivars
Open Access
- 6 October 2017
- Vol. 8 (10), 261
- https://doi.org/10.3390/genes8100261
Abstract
Rubber tree (Hevea brasiliensis) is the only commercially cultivated plant for producing natural rubber, one of the most essential industrial raw materials. Knowledge of the evolutionary and functional characteristics of kinases in H. brasiliensis is limited because of the long growth period and lack of well annotated genome information. Here, we reported mitogen-activated protein kinases in H. brasiliensis (HbMPKs) by manually checking and correcting the rubber tree genome. Of the 20 identified HbMPKs, four members were validated by proteomic data. Protein motif and phylogenetic analyses classified these members into four known groups comprising Thr-Glu-Tyr (TEY) and Thr-Asp-Tyr (TDY) domains, respectively. Evolutionary and syntenic analyses suggested four duplication events: HbMPK3/HbMPK6, HbMPK8/HbMPK9/HbMPK15, HbMPK10/HbMPK12 and HbMPK11/HbMPK16/HbMPK19. Expression profiling of the identified HbMPKs in roots, stems, leaves and latex obtained from three cultivars with different latex yield ability revealed tissue- and variety-expression specificity of HbMPK paralogues. Gene expression patterns under osmotic, oxidative, salt and cold stresses, combined with cis-element distribution analyses, indicated different regulation patterns of HbMPK paralogues. Further, Ka/Ks and Tajima analyses suggested an accelerated evolutionary rate in paralogues HbMPK10/12. These results revealed HbMPKs have diverse functions in natural rubber biosynthesis, and highlighted the potential possibility of using MPKs to improve stress tolerance in future rubber tree breeding.This publication has 56 references indexed in Scilit:
- Draft genome sequence of the rubber tree Hevea brasiliensisBMC Genomics, 2013
- ExPASy: SIB bioinformatics resource portalNucleic Acids Research, 2012
- MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony MethodsMolecular Biology and Evolution, 2011
- Mitogen-Activated Protein Kinase Signaling in PlantsAnnual Review of Plant Biology, 2010
- Circos: An information aesthetic for comparative genomicsGenome Research, 2009
- DnaSP v5: a software for comprehensive analysis of DNA polymorphism dataBioinformatics, 2009
- Arabidopsis MAPKs: a complex signalling network involved in multiple biological processesBiochemical Journal, 2008
- Clustal W and Clustal X version 2.0Bioinformatics, 2007
- MEME: discovering and analyzing DNA and protein sequence motifsNucleic Acids Research, 2006
- MAP kinase signalling cascade in Arabidopsis innate immunityNature, 2002