ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics
Open Access
- 8 April 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Genome Biology
- Vol. 22 (1), 1-34
- https://doi.org/10.1186/s13059-021-02302-5
Abstract
Single-cell sequencing technologies are revolutionizing biology, but they are limited by the need to dissociate live samples. Here, we present ACME (ACetic-MEthanol), a dissociation approach for single-cell transcriptomics that simultaneously fixes cells. ACME-dissociated cells have high RNA integrity, can be cryopreserved multiple times, and are sortable and permeable. As a proof of principle, we provide single-cell transcriptomic data of different species, using both droplet-based and combinatorial barcoding single-cell methods. ACME uses affordable reagents, can be done in most laboratories and even in the field, and thus will accelerate our knowledge of cell types across the tree of life.Keywords
Funding Information
- Medical Research Council (MR/S007849/1)
- The Royal Society (RGS\R1\191278)
- European Molecular Biology Organization (ALTF-217-2018)
- Biotechnology and Biological Sciences Research Council (BB/M011224/1)
- Oxford Brookes University
- Osk. Huttusen säätiö
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