Changing cell mechanics—a precondition for malignant transformation of oral squamous carcinoma cells
- 27 June 2018
- journal article
- research article
- Published by IOP Publishing in Convergent Science Physical Oncology
- Vol. 4 (3), 034001
- https://doi.org/10.1088/2057-1739/aac72d
Abstract
Oral squamous cell carcinomas (OSCC) are the sixth most common cancer and the diagnosis is often belated for a curative treatment. The reliable and early differentiation between healthy and diseased cells is the main aim of this study in order to improve the quality of the treatment and to understand tumour pathogenesis. Here, the optical stretcher is used to analyse mechanical properties of cells and their potential to serve as a marker for malignancy. Stretching experiments revealed for the first time that cells of primary OSCCs were deformed by 2.9% rendering them softer than cells of healthy mucosa which were deformed only by 1.9%. Furthermore, the relaxation behaviour of the cells revealed that these malignant cells exhibit a faster contraction than their benign counterparts. This suggests that deformability as well as relaxation behaviour can be used as distinct parameters to evaluate emerging differences between these benign and malignant cells. Since many studies in cancer research are performed with cancer cell lines rather than primary cells, we have compared the deformability and relaxation of both types, showing that long time culturing leads to softening of cells. The higher degree of deformability and relaxation behaviour can enable cancer cells to traverse tissue emphasizing that changes in cell architecture may be a potential precondition for malignant transformation. Respecting the fact that even short culture times have an essential effect on the significance of the results, the use of primary cells for further research is recommended. The distinction between malignant and benign cells would enable an early confirmation of cancer diagnoses by testing cell samples of suspect oral lesions.Keywords
Funding Information
- Sächsische Aufbaubank (9889/1519)
- H2020 European Research Council (ERC-741350)
- European Fund for Regional Development (EFRE) and the state of Saxony
This publication has 35 references indexed in Scilit:
- Are biomechanical changes necessary for tumour progression?Nature Physics, 2010
- Oral Cancer Diagnosis by Mechanical PhenotypingCancer Research, 2009
- Critical evaluation of diagnostic aids for the detection of oral cancerOral Oncology, 2008
- Quantifying the contribution of actin networks to the elastic strength of fibroblastsJournal of Theoretical Biology, 2006
- Prognostic relevance of cell proliferation in head and neck tumorsAnnals of Oncology, 2004
- Sphingosylphosphorylcholine regulates keratin network architecture and visco-elastic properties of human cancer cellsNature, 2003
- Tumor Cell Proliferation Kinetics and Tumor Growth RateActa Oncologica, 1989
- Two new human tumor cell lines derived from squamous cell carcinomas of the tongue: Establishment, characterization and response to cytotoxic treatmentEuropean Journal of Cancer and Clinical Oncology, 1988
- Relation between the pathological nature and the growth rate of human tumorsEuropean Journal of Cancer (1965), 1971
- On a Test of Whether one of Two Random Variables is Stochastically Larger than the OtherThe Annals of Mathematical Statistics, 1947