Benefits of Tiwai Onion (Eleutherine americana) Extract as Phytopharmaceutical Plant to Inhibit the Growth of Vibrio harveyi Through in-Vitro and in-Vivo
Open Access
- 21 March 2020
- journal article
- Published by Universitas Airlangga in Jurnal Ilmiah Perikanan dan Kelautan
- Vol. 12 (1), 105-112
- https://doi.org/10.20473/jipk.v12i1.12826
Abstract
Highlights Eleutherine americana was a plant that was known to contain antibacterial alkaloids, steroids, phenolics, and flavonoids Eleutherine americana extract was able to inhibit the development of V. harveyi both through in vitro and in vivo tests on tiger shrimp (Penaeus monodon). The use of vibriosis antibiotics in tiger shrimp was often not controlled so the results obtained were not effective Abstract The use of vibriosis antibiotics in tiger shrimp was often not controlled, so the results obtained were not effective. The addition of antibiotics would cause resistance to V. harveyi. Eleutherine americana is a plant that is known to contain antibacterial alkaloids, steroids, phenols, and flavonoids. This study aimed to determine the effectiveness of inhibitory effects of E. americana extract against V. harveyi through in-vitro and in-vivo tests on tiger shrimp larvae. In-vitro testing consisted of 7 treatments and 3 replications, namely treatments A (0.1%), B (0.2%), C (0.3%), D (0.4%), E (0, 5%), F ethanol 70% (K-), and G chloramphenicol 0.01% (K +) treatment. The largest inhibition zone diameter of E. americana extract was shown in treatment C (0.3%), with an average value of the inhibition zone produced of 7.5 mm. Challenge test with V. harveyi concentration of 107 CFU / ml in the in-vivo test consisted of 5 treatments and four replications namely; A treatment without E. americana extract, B extract 6 ppm, C extract 12 ppm, D extract 18 ppm, and treatment E without extract and V. harveyi. The results of the challenge test with V. harveyi bacteria were significantly different in control (chloramphenicol 0.01%), where the highest survival rate was in the treatment of 12 ppm extract (43.34%). E. americana extract could inhibit the development of V. harveyi bacteria both through in-vitro and in-vivo tests on tiger shrimp (Penaeus monodon).Keywords
This publication has 5 references indexed in Scilit:
- Uji daya hambat ekstrak bunga cengkeh (Syzygium aromaticum) terhadap bakteri Porphyromonas gingivalise-GiGi, 2016
- Antidermatophyte and antimelanogenesis compound from Eleutherine americana grown in IndonesiaJournal of Natural Medicines, 2010
- Chemical composition of the mangrove hollyAcanthus ilicifolius (Acanthaceae) — review and additional dataSenckenbergiana maritima, 2008
- Chemical Ecology of InsectsPublished by Springer Science and Business Media LLC ,1984
- Disc Plate Method of Microbiological Antibiotic AssayApplied and Environmental Microbiology, 1971