Stimulatory effect of the substance p antagonist spantide-II on aldosterone secretion of dispersed rat zona glomerulosa cells
- 1 January 1997
- journal article
- research article
- Published by Taylor & Francis Ltd in Endocrine Research
- Vol. 23 (1-2), 131-139
- https://doi.org/10.1080/07435809709031848
Abstract
Substance P (SP) did not affect either basal or agonist-stimulated aldosterone production by dispersed rat zona glomerulosa (ZG) cells. In contrast, the SP-receptor antagonist spantide-II (SPA), at 10−8/10−6 M concentrations, markedly raised basal and 10−9 M ACTH, but not 10−9 M angiotensin II-stimulated aldosterone secretion. The secretagogue effect of 10−6 M SPA was annulled by SP (10−6 M) and the protein kinase (PK)-C inhibitor Ro31-8220 (10−6M), but was unaffected by the PKA inhibitor H-89 (10−5M) In light of these findings the following conclusions can be drawn: (i) SP does not exert a physiologically relevant direct modulatory action on aldosterone secretion of rat ZG cells. (ii) a receptor-independent inhibitory interaction is likely to occur between SP and SPA molecules: and (iii) SPA activates, through a receptor-independent mechanism, phosphoinositide signaling pathway in rat ZG cells.Keywords
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