Rapid Screening Electrochemical Methods for Aflatoxin B1and Type‐A Trichothecenes: A Preliminary Study
- 31 May 2007
- journal article
- research article
- Published by Taylor & Francis Ltd in Analytical Letters
- Vol. 40 (7), 1333-1346
- https://doi.org/10.1080/00032710701326692
Abstract
In this work are presented methods for detection of aflatoxin B1 and type‐A trichothecenes, based on the use of indirect competitive ELISA format coupled with a 96‐well screen‐printed microplate. Electrochemical immunoassays for AFB1, T‐2, and HT‐2 were performed and the activity of the alkaline phosphatase or horseradish peroxidase labeled enzymes were measured using intermittent pulse amperometry (IPA) as electrochemical technique. Using standard solutions of the target analyte the LOD of the assays were 0.3 and 0.2 ng ml−1 for T‐2 and AFB1 respectively, while the sensitivity was 1.2 ng ml−1 for both. For Aflatoxin B1, a stability study of electrochemical plate was also performed. Moreover, the matrix effect was evaluated using two different extraction treatments from corn.Keywords
This publication has 16 references indexed in Scilit:
- Electrochemical immunosensor array using a 96-well screen-printed microplate for aflatoxin B1 detectionBiosensors and Bioelectronics, 2007
- Detection of Aflatoxin B1in Barley: Comparative Study of Immunosensor and HPLCAnalytical Letters, 2006
- Gas chromatography: mass spectrometry determination of trichothecene mycotoxins in commercial corn harvested in the state of São Paulo, BrazilJournal of the Brazilian Chemical Society, 2006
- A new solid phase extraction clean‐up method for the determination of 12 type A and B trichothecenes in cereals and cereal‐based food by LC‐MS/MSMolecular Nutrition & Food Research, 2006
- State of the art of trichothecenes analysisToxicology Letters, 2004
- Electrochemical immunosensor for determination of aflatoxin B1 in barleyAnalytica Chimica Acta, 2004
- Determination of trichothecenes in beer by capillary gas chromatography with flame ionisation detectionFood Chemistry, 2003
- MycotoxinsClinical Microbiology Reviews, 2003
- New strategies for the screening and determination of aflatoxins and the detection of aflatoxin-producing moulds in food and feedTrAC Trends in Analytical Chemistry, 2002
- Survey of T‐2 toxin in cereals by an indirect enzyme‐linked immunosorbent assayFood and Agricultural Immunology, 1990