Zinc oxide nanoparticles conjugated with clinically-approved medicines as potential antibacterial molecules
Open Access
- 10 July 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in AMB Express
- Vol. 11 (1), 1-16
- https://doi.org/10.1186/s13568-021-01261-1
Abstract
At present, antibiotic resistance is one of the most pressing issues in healthcare globally. The development of new medicine for clinical applications is significantly less than the emergence of multiple drug-resistant bacteria, thus modification of existing medicines is a useful avenue. Among several approaches, nanomedicine is considered of potential therapeutic value. Herein, we have synthesized Zinc oxide nanoparticles (ZnO-NPs) conjugated with clinically-approved drugs (Quercetin, Ceftriaxone, Ampicillin, Naringin and Amphotericin B) with the aim to evaluate their antibacterial activity against several Gram-positive (Methicillin resistant Staphylococcus aureus, Streptococcus pneumoniae and Streptococcus pyogenes) and Gram-negative (Escherichia coli K1, Serratia marcescens and Pseudomonas aeruginosa) bacteria. The nanoparticles and their drug conjugates were characterized using UV-visible spectrophotometry, dynamic light scattering, Fourier transform infrared spectroscopy and atomic force microscopy. Antibacterial activity was performed by dilution colony forming unit method and finally 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were performed to determine their cytotoxic effects against human cell lines. ZnO-NPs revealed maxima surface plasmon resonance band at 374 and after conjugation with beta-cyclodextrin at 379 nm, polydispersity with size in range of 25–45 nm with pointed shaped morphology. When conjugated with ZnO-NPs, drug efficacy against MDR bacteria was enhanced significantly. In particular, Ceftriaxone- and Ampicillin-conjugated ZnO-NPs exhibited potent antibacterial effects. Conversely, ZnO-NPs and drugs conjugated NPs showed negligible cytotoxicity against human cell lines except Amphotericin B (57% host cell death) and Amphotericin B-conjugated with ZnO-NPs (37% host cell death). In conclusion, the results revealed that drugs loaded on ZnO-NPs offer a promising approach to combat increasingly resistant bacterial infections.Keywords
Funding Information
- American University of Sharjah
- University of Sharjah
This publication has 70 references indexed in Scilit:
- Antimicrobial activity of metal oxide nanoparticles against Gram-positive and Gram-negative bacteria: a comparative studyInternational Journal of Nanomedicine, 2012
- Antimicrobial applications of nanotechnology: methods and literatureInternational Journal of Nanomedicine, 2012
- Synthesis and antibacterial activity of silver nanoparticles against gram-positive and gram-negative bacteriaNanomedicine: Nanotechnology, Biology and Medicine, 2012
- AmpC β-LactamasesClinical Microbiology Reviews, 2009
- Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganismsFEMS Microbiology Letters, 2008
- Ampicillin/SulbactamDrugs, 2007
- Encapsulation of amphotericin B in poly(ethylene glycol)-block-poly(ɛ-caprolactone-co-trimethylenecarbonate) polymeric micellesInternational Journal of Pharmaceutics, 2006
- CeftriaxoneDrugs, 2002
- Mode of action of β-lactam antibioticsPharmacology & Therapeutics, 1985
- Antibiotic resistance in Serratia marcescens.BMJ, 1983