A Case Report of Decreased Hatching Rate of Eggs Caused by Co-Infection of Escherichia coli and Klebsiella

Abstract

本文报告了一例大肠杆菌和克雷伯氏菌共感染导致鸡群种蛋孵化率降低的病例，内容包括细菌的分离鉴定、药敏试验和鸡胚致死率试验。结果显示，出现种蛋孵化率降低的种蛋死胚中同时分离到大肠杆菌和克雷伯氏菌，两种细菌分离率明显高于正常孵化率的种蛋死胚，且种蛋孵化率降低的种鸡肛拭子中大肠杆菌和克雷伯氏菌的分离率也明显高于孵化率正常的种鸡，据此确诊为种鸡大肠杆菌和克雷伯氏菌共感染造成的鸡胚孵化率降低。通过药敏试验筛选对上述两种细菌均敏感的药物进行治疗后种蛋孵化率逐渐得以恢复；采用鸡胚致死率试验发现大肠杆菌和克雷伯氏菌共感染较两种菌单独感染引起的鸡胚死亡速度更快、死亡率更高。 This paper reports a case of co-infection of Escherichia coli and Klebsiella causing decreased hatch-ing rate of chicken eggs, including isolation and identification of bacteria, drug sensitivity test and chicken embryo lethal test. The results showed that Escherichia coli and Klebsiella were simultaneously isolated from fresh dead embryos with reduced hatching rate, and the bacterial isolation rate was significantly higher than that of dead embryos with normal hatching rate. Moreover, the isolation rates of Escherichia coli and Klebsiella were significantly higher in anal swabs from breeder with reduced hatching rate than the normal. Based on the results, the decreased hatching rate of chicken eggs was diagnosed with co-infection of Escherichia coli and Klebsiella in breeder. After treatment with the sensitive antibiotics to these two kinds of bacteria determinated by susceptibility testing, and embryo hatching rate gradually returns to normal level. The chicken embryo lethality test showed that chicken embryos co-infected with Escherichia coli and Klebsiella simultaneously died faster and had higher mortality rates than those of the infection alone of two kinds of bacteria.