Assessment of Cholesteryl Ester Transfer Protein Gene Taq1B Polymorphism and Its Relationship with Lipid Levels of Hypertensive Iraqi Patients

Abstract

Lipid disorder is one of the main causes of essential hypertension. Cholesteryl ester transfer protein (CETP) gene polymorphism is known to be associated with changes in lipid profile. Therefore, this study designed to detect the CETP Taq1 B polymorphism (rs708272) and evaluate its relationship with lipid profile levels among some hypertensive Iraqi patients. One hundred and seventy blood samples were collected from two groups, the first group included a hundred hypertensive patients and the second group included seventy healthy individuals as control group. For both groups, lipid profile was estimated, genomic DNA was extracted from all samples and Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) technique was used to detect CETP gene Taq1 B polymorphism. Results indicated that levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL-C), and very-low-density lipoprotein (VLDL-C) had increased, while the level of high-density lipoprotein (HDL-C) was decreased in hypertensive patients in comparison to control group. The study also showed that the frequency of B1B1 genotype and (B1) allele was higher in hypertensive patients in compare with control group (p ≤ 0.01). Lipid profile concentrations according to CETP gene Taq1 B genotypes showed non-significant differences. This study concluded that the CETP gene Taq1B polymorphism may associated with associated with lipid disorder and CETP (B1) allele which could be used as a genetic marker for increasing hypertensive susceptibility in Iraqi population.