Objective: To determine the relationship of Chlamydia trachomatis infection in patient with ruptured ectopic pregnancy through examination of endocervical swabs, tubal tissue using Polymerase chain reaction (PCR) and Enzyme linked immunoabsorb and assay (ELISA) serum IgG antibodies Specific to Chlamydia trachomatis.Method: This study was an observational analytic study with a cross sectional study design. In this study, there were 50 participants consisting of 25 ruptured ectopic pregnancy patients and 25 non-ruptured ectopic pregnancy patients who underwent treatment at Dr.Wahidin Sudirohusodo Hospital as well as networking hospitals at the Universitas Hasanuddin in Makassar City.Results: The results showed that Chlamydia trachomatis infection in patients with ruptured ectopic pregnancies was found to be 84% positive in tubal tissue, 72% with endocervical swabs and 64% with serum examination. There was a significant relationship between chlamydial tracheal infection obtained through examination of tubal tissue, endocervical swab and specific serum IgG in patients with ruptured ectopic pregnancy (p