LncRNA NEAT1 controls the lineage fates of BMSCs during skeletal aging by impairing mitochondrial function and pluripotency maintenance
Open Access
- 8 September 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Cell Death & Differentiation
- Vol. 29 (2), 351-365
- https://doi.org/10.1038/s41418-021-00858-0
Abstract
Aged bone marrow mesenchymal stem cells (BMSCs) exhibit aberrant self-renewal and lineage specification, which contribute to imbalanced bone-fat and progressive bone loss. In addition to known master regulators of lineage commitment, it is crucial to identify pivotal switches governing the specific differentiation fate of aged BMSCs. Here, we profiled differences in epigenetic regulation between adipogenesis and osteogenesis and identified super-enhancer associated lncRNA nuclear-enriched abundant transcript 1 (NEAT1) as a key bone-fat switch in aged BMSCs. We validated that NEAT1 with high enhancer activity was transcriptionally activated by ATF2 and directed aged BMSCs to a greater propensity to differentiate toward adipocytes than osteoblasts by mediating mitochondrial function. Furthermore, we confirmed NEAT1 as a protein-binding scaffold in which phosphorylation modification of SOX2 Ser249/250 by CDK2 impaired SOX2/OCT4 complex stability and dysregulated downstream transcription networks of pluripotency maintenance. In addition, by sponging miR-27b-3p, NEAT1 upregulated BNIP3L, BMP2K, and PPARG expression to shape mitochondrial function and osteogenic/adipogenic differentiation commitment, respectively. In extracellular communication, NEAT1 promoted CSF1 secretion from aged BMSCs and then strengthened osteoclastic differentiation by extracellular vesicle delivery. Notably, Neat1 small interfering RNA delivery induced increased bone mass in aged mice and decreased fat accumulation in the bone marrow. These findings suggest that NEAT1 regulates the lineage fates of BMSCs by orchestrating mitochondrial function and pluripotency maintenance, and might be a potential therapeutic target for skeletal aging.This publication has 62 references indexed in Scilit:
- PKCε Promotes Oncogenic Functions of ATF2 in the Nucleus while Blocking Its Apoptotic Function at MitochondriaCell, 2012
- A network connecting Runx2, SATB2, and the miR-23a∼27a∼24-2 cluster regulates the osteoblast differentiation programProceedings of the National Academy of Sciences of the United States of America, 2010
- PPARγ: a circadian transcription factor in adipogenesis and osteogenesisNature Reviews Endocrinology, 2010
- An Oct4-Centered Protein Interaction Network in Embryonic Stem CellsCell Stem Cell, 2010
- Emerging roles of ATF2 and the dynamic AP1 network in cancerNature Reviews Cancer, 2010
- Control of Stem Cell Fate by Physical Interactions with the Extracellular MatrixCell Stem Cell, 2009
- An Architectural Role for a Nuclear Noncoding RNA: NEAT1 RNA Is Essential for the Structure of ParaspecklesMolecular Cell, 2009
- Transcriptional control of adipocyte formationCell Metabolism, 2006
- The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cellsNature Genetics, 2006
- Core Transcriptional Regulatory Circuitry in Human Embryonic Stem CellsCell, 2005