Inulinase production capability of a promising medicinal plant: Inula viscosa
Open Access
- 30 June 2020
- journal article
- Published by Commagene Journal of Biology in Commagene Journal of Biology
- Vol. 4 (1), 67-73
- https://doi.org/10.31594/commagene.747618
Abstract
En tr The present study was designed to examine the inulinase production capability of Rhodotorula glutinis SO-28 by using Inula viscosa, a promising medicinal plant, as sole carbon source in submerged fermentation. Inula viscosa, a perennial member of Asteraceae family, is a popular and widespread medicinal plant in the Mediterranean region. It is termed as “yapışkan andız otu” in Turkey and has been widely used in folk medicine since the ancient times. Taguchi design of experiment (DOE) technique was utilized for the inulinase production optimization process. An orthogonal array layout of L16 was utilized with four influential factors as following: Inula viscosa amount, agitation speed, incubation temperature, and incubation time at four levels. The obtained results showed that optimized inulinase production enhanced enzyme activity as 99.63 U/ml which was 5-fold higher than the unoptimized condition. In brief, Inula viscosa can be used effectively for inulinase production and use of statistical optimization techniques like Taguchi DOE significantly increases the enzyme yield. Bu çalışma, batık kültür fermantasyonunda tek karbon kaynağı olarak umut verici bir tıbbi bitki olan Inula viscosa kullanılarak Rhodotorula glutinis SO-28'in inulinaz üretim kapasitesini incelemek üzere tasarlanmıştır. Asteraceae familyasının çok yıllık bir üyesi olan Inula viscosa, Akdeniz bölgesinde popüler ve yaygın bir tıbbi bitkidir. Türkiye'de “yapışkan andız otu” olarak adlandırılmakta ve eski çağlardan beri halk tıbbında yaygın olarak kullanılmaktadır. İnülinaz üretim optimizasyonu sürecinde Taguchi deney tasarımı (DOE) tekniği kullanılmıştır. Inula viscosa miktarı, çalkalama hızı, inkübasyon sıcaklığı ve inkübasyon süresi olmak üzere dört etkin faktörlü ve dört seviyeli olan L16 ortogonal dizilim kullanılmıştır. Elde edilen sonuçlar, optimize edilmiş inulinaz üretiminin enzim aktivitesini, optimize edilmemiş durumdan 5 kat daha yüksek olan 99.63 U / ml olarak arttırdığını göstermiştir. Kısacası Inula viscosa, inülinaz üretiminde etkin bir şekilde kullanılabilir ve Taguchi DOE gibi istatistiksel optimizasyon tekniklerinin kullanımı enzim verimini azımsanmayacak ölçüde artırmaktadır.Keywords
This publication has 44 references indexed in Scilit:
- Influence of the shape and mass of a small robot when thrown to a dummy human headSN Applied Sciences, 2019
- Inula Viscosa Extract Inhibits Growth of Colorectal Cancer Cells in vitro and in vivo Through Induction of ApoptosisFrontiers in Oncology, 2019
- Isocostic Acid, a Promising Bioactive Agent from the Essential Oil of Inula viscosa (L.): Insights from Drug Likeness Properties, Molecular Docking and SAR AnalysisChemistry & Biodiversity, 2019
- A review of the most effective medicinal plants for dermatophytosis in traditional medicineBiomedical Research and Therapy, 2018
- Tris–sucrose buffer system: a new specially designed medium for extracellular invertase production by immobilized cells of isolated yeast Cryptococcus laurentii MT-61Folia Microbiologica, 2013
- Ethnopharmacological survey of wild medicinal plants in Showbak, JordanJournal of Ethnopharmacology, 2009
- Effects of Inula viscosa leaf extracts on abortion and implantation in ratsJournal of Ethnopharmacology, 2001
- Ethnobotanical survey in the Palestinian area: a classification of the healing potential of medicinal plantsJournal of Ethnopharmacology, 2000
- Pharmacological screening of the anti-ulcerogenic effects of some Jordanian medicinal plants in ratsJournal of Ethnopharmacology, 1999
- A New Eudesmane Acid fromDittrichia (Inula) viscosaPlanta Medica, 1985