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Multiplikasi Pucuk pada Tanaman Doyo (Curculigo latifolia Dryand.) Menggunakan Beberapa Kombinasi BAP dan IBA pada Perbanyakan In-Vitro

Aswan Efendi, Widi Sunaryo, Nurhasanah Nurhasanah

Abstract:The development of the Ulap Doyo weaving industry in East Kalimantan is constrained by doyo plants availability as raw material. Cultivation of Doyo or Marasi plant conventionally faces obstacles due to the low germination rate and deteriotation of doyo seeds. Tissue culture is considered as an important technology for mass propagation producing disease-free, high quality, uniform and rapid production of planting material. This study aims to induce regeneration and increase the multiplication of doyo plant for mass multiplication to support the doyo plant conservation program. The research was conducted in 3 stages: shoot initiation, shoot multiplication and plant regeneration (root induction). Shoot initiation and multiplication experiments were carried out using several combinations of 6-Benzylaminopurine (BAP) and Indole-3-butyric acid (IBA) in solid MS media, and two explants types, shoots and rhizomes. The root induction was carried out by growing the plants in MS media containing 0.25 mg L-1 IBA. The results showed that the rhizome was the best part of the plant as a source of explants in doyo plant tissue culture. Initiation medium of 3.75 mg L-1 BAP + 0.50 mg L-1 IBA and the multiplication medium of  37.50 mg L-1 BAP + 0.50 mg L-1 IBA, gave the best effect resulting in the highest survival and responsiveness percentage (80%), percentage of shoot producing explants( 60%, and number of shoots per explant ( 4.00). The plant regeneration has good potential for doyo plant mass propagation, in which 87.50% of the resulting shoots were able to form good root system.
Keywords: IBA / BAP / sup / doyo plant / tissue culture / plant regeneration / mass propagation

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