Expression of PAR2 and NF-κB in human primary dental pulp odontoblasts during the progression of caries
- 6 May 2022
- journal article
- Published by Peertechz Publications Private Limited
- Vol. 5 (1), 001-004
- https://doi.org/10.17352/ojcps.000004
Abstract
Increased proteinase-activated receptor-2 (PAR2) expression is observed in various diseases related to inflammation. However, the expression of PAR2 in odontoblasts in response to dental caries has not been investigated. Therefore, to explore the functions of odontoblasts during the progression of carious infection, we measured PAR2 and NF-κB expression using immunofluorescence techniques in the odontoblast layer and pulpocytes in the sub-odontoblast region of 44 teeth extracted from children undergoing dental treatment (eight sound samples, 13 early carious samples, 16 advanced carious samples, and eight exposed pulp samples). PAR2 and NF-κB were expressed at moderate levels in sound teeth with non-carious pulp, and the expression levels changed as caries progressed. PAR2 was significantly upregulated in the odontoblast layer during early-stage and advanced-stage caries, and reduced below healthy levels in teeth with exposed pulp. NF-κB was significantly upregulated in early-stage caries and significantly downregulated in advanced-stage and late-stage caries. Moreover, in the sub-odontoblast region, NF-κB expression increased with the progression of caries. Overall, this study suggests PAR2 may represent a crucial cell signalling receptor in the dentine-pulp complex during dental inflammation, and that NF-κB may be one of the key pathways that regulate inflammatory immune responses in the dental pulp.Keywords
This publication has 24 references indexed in Scilit:
- Cytokines and chemokines: At the crossroads of cell signalling and inflammatory diseaseBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2014
- Periodontal Treatment Downregulates Protease-Activated Receptor 2 in Human Gingival Crevicular Fluid CellsInfection and Immunity, 2013
- Human odontoblasts express functional thermo-sensitive TRP channels: Implications for dentin sensitivityPain, 2011
- Cleavage of protease-activated receptors on an immortalized oral epithelial cell line by Porphyromonas gingivalis gingipainsMicrobiology, 2009
- Protease-activated receptor-2 activation: a major actor in intestinal inflammationGut, 2008
- Cytokine upregulation of proteinase‐activated‐receptors 2 and 4 expression mediated by p38 MAP kinase and inhibitory kappa B kinase β in human endothelial cellsBritish Journal of Pharmacology, 2007
- Mast Cell Tryptase Controls Paracellular Permeability of the IntestineOnline Journal of Public Health Informatics, 2005
- Parallels between Tooth Development and Repair: Conserved Molecular Mechanisms following Carious and Dental InjuryJournal of Dental Research, 2004
- Altered expression and in vivo lung function of protease-activated receptors during influenza A virus infection in miceAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2004
- Differential Expression of Protease-Activated Receptors-1 and -2 in Stromal Fibroblasts of Normal, Benign, and Malignant Human TissuesThe American Journal of Pathology, 2001