New Search

Advanced search
Export article
Open Access

A Microfluidic Platform Based on Robust Gas and Liquid Exchange for Long-term Culturing of Explanted Tissues.

Nobutoshi Ota, Genki N. Kanda, Hiroyuki Moriguchi, Yusufu Aishan, Yigang Shen, Rikuhiro G. Yamada, Hiroki R. Ueda, Sciprofile linkYo Tanaka
Analytical Sciences , Volume 35, pp 1141-1147; doi:10.2116/analsci.19P099

Abstract: Microfluidic devices are important platforms to culture and observe biological tissues. Compared with conventional setups, microfluidic devices have advantages in perfusion, including an enhanced delivery of nutrients and gases to tissues. However, explanted tissues can maintain their functions for only hours to days in microfluidic devices, although their observations are desired for weeks. The suprachiasmatic nucleus (SCN) is a brain region composed of heterogeneous cells to control the biological clock system through synchronizing individual cells in this region. The synchronized and complicated cell-cell interactions of SCN cells are difficult to reproduce from seeded cells. Thus, the viability of explanted SCN contributes to the study of SCN functions. In this paper, we propose a new perfusion platform combining a PDMS microfluidic device with a porous membrane to culture an explanted SCN for 25 days. We expect that this platform will provide a universal interface for microfluidic manipulation of tissue explants.
Keywords: circadian rhythm / Brain slice / porous membrane / suprachiasmatic nucleus / Organotypic culture

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

Share this article

Click here to see the statistics on "Analytical Sciences" .
Cited by 1 articles
    Back to Top Top