A Microfluidic Platform Based on Robust Gas and Liquid Exchange for Long-term Culturing of Explanted Tissues
Open Access
- 10 October 2019
- journal article
- research article
- Published by Springer Science and Business Media LLC in Analytical Sciences
- Vol. 35 (10), 1141-1147
- https://doi.org/10.2116/analsci.19p099
Abstract
Microfluidic devices are important platforms to culture and observe biological tissues. Compared with conventional setups, microfluidic devices have advantages in perfusion, including an enhanced delivery of nutrients and gases to tissues. However, explanted tissues can maintain their functions for only hours to days in microfluidic devices, although their observations are desired for weeks. The suprachiasmatic nucleus (SCN) is a brain region composed of heterogeneous cells to control the biological clock system through synchronizing individual cells in this region. The synchronized and complicated cell–cell interactions of SCN cells are difficult to reproduce from seeded cells. Thus, the viability of explanted SCN contributes to the study of SCN functions. In this paper, we propose a new perfusion platform combining a PDMS microfluidic device with a porous membrane to culture an explanted SCN for 25 days. We expect that this platform will provide a universal interface for microfluidic manipulation of tissue explants.Keywords
This publication has 24 references indexed in Scilit:
- Recent Progress in the Development of Microfluidic Vascular ModelsAnalytical Sciences, 2018
- Powering ex vivo tissue models in microfluidic systemsLab on a Chip, 2018
- Microsensor systems for cell metabolism – from 2D culture to organ-on-chipLab on a Chip, 2018
- Regulating the Suprachiasmatic Nucleus (SCN) Circadian Clockwork: Interplay between Cell-Autonomous and Circuit-Level MechanismsCold Spring Harbor Perspectives in Biology, 2017
- High Temporal Resolution Detection of Patient-Specific Glucose Uptake from Human ex Vivo Adipose Tissue On-ChipAnalytical Chemistry, 2015
- Mice Genetically Deficient in Vasopressin V1a and V1b Receptors Are Resistant to Jet LagScience, 2013
- Brain slice on a chip: opportunities and challenges of applying microfluidic technology to intact tissuesLab on a Chip, 2012
- Chamber and microfluidic probe for microperfusion of organotypic brain slicesLab on a Chip, 2009
- PERIOD2::LUCIFERASE real-time reporting of circadian dynamics reveals persistent circadian oscillations in mouse peripheral tissuesProceedings of the National Academy of Sciences of the United States of America, 2004
- The VPAC2 Receptor Is Essential for Circadian Function in the Mouse Suprachiasmatic NucleiCell, 2002