Viral Manipulation of a Mechanoresponsive Signaling Axis Disassembles Processing Bodies
Open Access
- 13 September 2021
- journal article
- research article
- Published by Taylor & Francis Ltd in Molecular and Cellular Biology
- Vol. 41 (11), e0039921
- https://doi.org/10.1128/mcb.00399-21
Abstract
Processing bodies (PBs) are ribonucleoprotein granules important for cytokine mRNA decay that are targeted for disassembly by many viruses. Kaposi’s sarcoma-associated herpesvirus is the etiological agent of the inflammatory endothelial cancer, Kaposi’s sarcoma, and a PB-regulating virus. The virus encodes Kaposin B (KapB), which induces actin stress fibres (SFs) and cell spindling as well as PB disassembly. We now show that KapB-mediated PB disassembly requires actin rearrangements, RhoA effectors and the mechanoresponsive transcription activator, YAP. Moreover, ectopic expression of active YAP or exposure of ECs to mechanical forces caused PB disassembly in the absence of KapB. We propose that the viral protein KapB activates a mechanoresponsive signaling axis and links changes in cell shape and cytoskeletal structures to enhanced inflammatory molecule expression using PB disassembly. Our work implies that cytoskeletal changes in other pathologies may similarly impact the inflammatory environment.Keywords
Funding Information
- Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (RGPIN-2015-04882)
This publication has 141 references indexed in Scilit:
- LSm14A is a processing body-associated sensor of viral nucleic acids that initiates cellular antiviral response in the early phase of viral infectionProceedings of the National Academy of Sciences of the United States of America, 2012
- β-Actin specifically controls cell growth, migration, and the G-actin poolMolecular Biology of the Cell, 2011
- Viral Latency and Its Regulation: Lessons from the γ-HerpesvirusesCell Host & Microbe, 2010
- Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamicsNature, 2010
- Elastic Moduli of Collagen Gels Can Be Predicted from Two-Dimensional Confocal MicroscopyBiophysical Journal, 2009
- Arachidonic Acid Stimulates Cell Adhesion through a Novel p38 MAPK-RhoA Signaling Pathway That Involves Heat Shock Protein 27Online Journal of Public Health Informatics, 2009
- Demonstration of catch bonds between an integrin and its ligandThe Journal of cell biology, 2009
- The Dynamics of Mammalian P Body Transport, Assembly, and Disassembly In VivoMolecular Biology of the Cell, 2008
- Stress fibers are generated by two distinct actin assembly mechanisms in motile cellsThe Journal of cell biology, 2006
- Mechanism of Blebbistatin Inhibition of Myosin IIOnline Journal of Public Health Informatics, 2004