Morphology and Chemical Phenotype of the Ovarian Intrinsic Neurons in Neonate and Sexually Mature Reproductive Guinea Pig
Open Access
- 1 January 2015
- journal article
- research article
- Published by Scientific Research Publishing, Inc. in Advances in Reproductive Sciences
- Vol. 03 (01), 13-26
- https://doi.org/10.4236/arsci.2015.31002
Abstract
Introduction: The existence of ovarian intrinsic neurons is well established. However, the morphology and chemical phenotype are not completely characterized and are even unknown for some species used in medical research. The purpose of this work was to determine the morphology and chemical phenotype of intrinsic neurons of the guinea pig ovary at two ages: neonates (0 days old) and sexually mature reproductive animals (90 days old). Materials and Methods: For the morphological analysis, we employed the modified Golgi-Cox impregnation technique. For the che- mical phenotype, we used immunohistochemistry and the following antibodies; tyrosine hydroxylase (TH), calcitonin gene-related peptide (CGRP), transient receptor potential type 1 (TRPV1), neuron-specific nuclear protein (NeuN) and proto-oncogene product of the cFos gene (cFos). We also used enzyme histochemistry for NADPH-diaphorase detection. Results: The number of intrinsic neurons in the neonate ovary was low in comparison to the adult guinea pig ovary. The intrinsic neurons were located in the cortex and the ovarian medulla; some were isolated or clustered, forming ganglia, and others were interconnected and formed networks. The neurons were small, medium or large. In the cortex of neonate vs adult ovaries, the small and medium neurons comprised 23% vs 36% and 5.2% vs 11.6%, respectively. In the medulla, the percent of the same neurons was 10.1% vs 10.1% and 1.1% vs 2.2% in the neonate and adult, respectively. In both cortex and medulla < 1% were large neurons at two ages. Also, the neurons were rounded, fusiform or multipolar. In the cortex, they were 12.7% vs 20.9%, 14.9% vs 24.2% and 1.1% vs 3.0%, respectively. In the medulla, the percent of small vs medium neurons was 6% vs 7.1% and 4.1% vs 4.8% in the neonate and adult ovary, respectively, and <1% were large neurons at both ages. The chemical phenotypes were in the neonate and adult: TH/NeuN-positive neurons, 16.3% vs 26.5%; CGRP/NeuN, 13.5% vs 35.8%; TRPV1/NeuN, 10.2% vs 38.6%; and cFos/NeuN, 4.6% vs 5.4%, respectively. The percent of NADPHd-positive cells in the cortex was 9.5% vs 25.1% and 3.2% vs 62.2% in the medulla in the neonate and adult, respectively. Conclusion: Altogether, these data showed that the number of ovarian intrinsic neurons was low at birth and increased in the sexually mature reproductive guinea pig. The chemical phenotype was rich and peptidergic, catecholaminergic and nitrergic in nature and positive for cFos immunoreactivity. Therefore, intrinsic neurons can be chemical sensors inside of the gonad and transmit signal to the central nervous system.Keywords
This publication has 36 references indexed in Scilit:
- Reduction of the Number of Neurones in the Caudal Mesenteric Ganglion Innervating the Ovary in Sexually Mature Gilts Following Testosterone AdministrationJournal of Neuroendocrinology, 2013
- Ovarian Surface Epithelium in Patients with Severe Ovarian Infertility: A Potential Source of Cells Expressing Markers of Pluripotent/Multipotent Stem CellsJournal of Biomedicine and Biotechnology, 2011
- Retraction: Detection, Characterization, and Spontaneous Differentiation In Vitro of Very Small Embryonic-Like Putative Stem Cells in Adult Mammalian OvaryStem Cells and Development, 2011
- Immunohistochemical analysis of substance P-containing neurons in rat small intestineCell and tissue research, 2010
- Immunohistochemical characteristics of submucosal Dogiel type II neurons in rat colonCell and tissue research, 2010
- Intrinsic innervation of the horse ileumResearch in Veterinary Science, 2009
- Modulation of motor and sensory pathways of the peristaltic reflex by cannabinoidsAmerican Journal of Physiology-Gastrointestinal and Liver Physiology, 2009
- Functional Development of the Ovarian Noradrenergic InnervationEndocrinology, 2007
- Critical Evaluation of the Colocalization Between Calcitonin Gene-Related Peptide, Substance P, Transient Receptor Potential Vanilloid Subfamily Type 1 Immunoreactivities, and Isolectin B4 Binding in Primary Afferent Neurons of the Rat and MouseThe Journal of Pain, 2007
- Primary afferent neurons intrinsic to the guinea-pig intestine, like primary afferent neurons of spinal and cranial sensory ganglia, bind the lectin, IB4Cell and tissue research, 2005