Effect of cAMP and cGMP on Connexin43 Expression in Isolated Human and Bovine Ciliary Epithelium
Open Access
- 1 January 2022
- journal article
- research article
- Published by Scientific Research Publishing, Inc. in American Journal of Molecular Biology
- Vol. 12 (03), 97-108
- https://doi.org/10.4236/ajmb.2022.123009
Abstract
The aim of the study was to assess the distribution of connexin43 (Cx43) and connexin40 (Cx40) in human and bovine ciliary bodies. The effect of the second messengers cAMP and cyclic cGMP on Cx43 protein expression was also investigated. Enucleated human eyes (remnant after corneal transplantation) and bovine eyes were used. Tissue preparations of the anterior segments of the eyes have proceeded for immunohistochemical staining with polyclonal antibodies of Cx43 and Cx40. Isolated ciliary bodies of human and bovine eyes were incubated with cAMP analog 8-Bromo-cAMP or the cGMP analog 8-Bromo-cGMP, the expression of Cx43 protein in the tissues was then assessed by Western blot assay. Both in human and bovine ciliary bodies, strong immunoreactivity of Cx43, but not Cx40, was observed predominantly in the apical cytoplasmic portions of the pigment ciliary epithelial cells and non-pigmented ciliary epithelial cells. In human ciliary body both cAMP and cGMP up-regulated Cx43 expression, while in the bovine ciliary body, cGMP increased Cx43 expression but cAMP decreased it. Cx43 is the major component of human and bovine gap junctions in the ciliary epithelium. The regulation on the Cx43 expression by cAMP and cGMP in human and bovine ciliary bodies suggests the possibly different roles of these signal messengers in the intracellular communication.Keywords
This publication has 46 references indexed in Scilit:
- Endogenous Production of Extracellular Adenosine by Trabecular Meshwork Cells: Potential Role in Outflow RegulationInvestigative Ophthalmology & Visual Science, 2012
- Gap-junction-mediated cell-to-cell communicationCell and tissue research, 2012
- Ciliary blood flow and aqueous humor productionProgress in Retinal and Eye Research, 2011
- Pathways for ATP release by bovine ciliary epithelial cells, the initial step in purinergic regulation of aqueous humor inflowAmerican Journal of Physiology-Cell Physiology, 2010
- Regulation of gap junction coupling in bovine ciliary epitheliumAmerican Journal of Physiology-Cell Physiology, 2010
- Morphine-induced nitric oxide production in isolated, iris-ciliary bodiesExperimental Eye Research, 2009
- Depression of Intraocular Pressure Following Inactivation of Connexin43 in the Nonpigmented Epithelium of the Ciliary BodyInvestigative Ophthalmology & Visual Science, 2009
- Connexin43 is required for production of the aqueous humor in the murine eyeJournal of Cell Science, 2006
- Protein Kinase A-Dependent Phosphorylation of Aquaporin-1Biochemical and Biophysical Research Communications, 2000
- Expression and cellular distribution of the al gap junction gene product in the ocular pigmented ciliary epitheliumCurrent Eye Research, 1992