This study aimed to evaluate the knowledge of health professionals and students about leishmaniasis in the region of the southern Pernambuco region. Data collection was performed between July and August 2016, based on the application of questionnaires to 94 health professionals and their analysis was based on descriptive and inferential statistics techniques. The association between the knowledge of leishmaniasis and health professionals was verified, although veterinarians have demonstrated greater knowledge of leishmaniasis compared to other professionals interviewed in the health area at a significant level of p 0.05.