Investigating Virus–Host Interactions in Cultured Primary Honey Bee Cells
Open Access
- 17 July 2021
- Vol. 12 (7), 653
- https://doi.org/10.3390/insects12070653
Abstract
Honey bee (Apis mellifera) health is impacted by viral infections at the colony, individual bee, and cellular levels. To investigate honey bee antiviral defense mechanisms at the cellular level we further developed the use of cultured primary cells, derived from either larvae or pupae, and demonstrated that these cells could be infected with a panel of viruses, including common honey bee infecting viruses (i.e., sacbrood virus (SBV) and deformed wing virus (DWV)) and an insect model virus, Flock House virus (FHV). Virus abundances were quantified over the course of infection. The production of infectious virions in cultured honey bee pupal cells was demonstrated by determining that naïve cells became infected after the transfer of deformed wing virus or Flock House virus from infected cell cultures. Initial characterization of the honey bee antiviral immune responses at the cellular level indicated that there were virus-specific responses, which included increased expression of bee antiviral protein-1 (GenBank: MF116383) in SBV-infected pupal cells and increased expression of argonaute-2 and dicer-like in FHV-infected hemocytes and pupal cells. Additional studies are required to further elucidate virus-specific honey bee antiviral defense mechanisms. The continued use of cultured primary honey bee cells for studies that involve multiple viruses will address this knowledge gap.Keywords
Funding Information
- Project Apis m. (not applicable)
- National Science Foundation (1651561, not applicable, NC-1173)
This publication has 192 references indexed in Scilit:
- Non-Target Effects of Green Fluorescent Protein (GFP)-Derived Double-Stranded RNA (dsRNA-GFP) Used in Honey Bee RNA Interference (RNAi) AssaysInsects, 2013
- Making connections in insect innate immunityProceedings of the National Academy of Sciences of the United States of America, 2012
- Viral Suppressors of RNA-Based Viral Immunity: Host TargetsCell Host & Microbe, 2010
- Virus discovery by deep sequencing and assembly of virus-derived small silencing RNAsProceedings of the National Academy of Sciences of the United States of America, 2010
- Alphavirus-derived small RNAs modulate pathogenesis in disease vector mosquitoesProceedings of the National Academy of Sciences of the United States of America, 2008
- Recent insights into the biology and biomedical applications of Flock House virusCellular and Molecular Life Sciences, 2008
- Virus infection causes specific learning deficits in honeybee foragersProceedings. Biological sciences, 2007
- A genomewide screen for components of the RNAi pathway in Drosophila cultured cellsProceedings of the National Academy of Sciences of the United States of America, 2006
- Essential function in vivo for Dicer-2 in host defense against RNA viruses in drosophilaNature Immunology, 2006
- Animal virus replication and RNAi-mediated antiviral silencing in Caenorhabditis elegansNature, 2005