EBV and HP Infections in Gastric Cancer Tissues and Their Correlation with HER2, P53, Ki-67 Gene Expression

Abstract

目的：评价EB病毒、幽门螺杆菌感染与胃癌组织中表皮生长HER2、P53、Ki-67表达的相关性，初步探讨两者感染在胃癌发生发展的相关作用。方法：收集我院行胃癌根治手术病例的组织蜡块共78例，采用原位杂交方法检测胃癌组织中EBV感染情况，利用免疫组织化学方法检测胃癌组织中Hp感染状态以及HER2、P53、Ki-67等蛋白表达情况。结果：EBV感染阳性率为12.8% (10/78)，Hp阳性率为57.7% (45/78)，EBV、Hp两者感染不存在一致性；胃癌组织中EBV、Hp感染分别与性别、年龄、淋巴结有无转移、胃腺癌分化程度、浸润深度、临床分期无显著差异；HER2、P53、Ki-67等蛋白表达在胃癌组织中与性别、年龄、淋巴结有无转移、腺癌分化程度、浸润深度、临床分期无显著差异；P53、Ki-67蛋白表达与Hp感染有显著性差异，P53、Ki-67蛋白表达与Hp感染存在正相关关系，Hp阳性组与Hp阴性组HER2蛋白表达无显著性差异；EBV阳性或Hp阳性组与两者联合感染组中P53、Ki-67蛋白表达差异无统计学意义。结论：Hp感染组与非感染组胃癌组织中P53、Ki-67表达存在显著差异，且Hp感染与P53、Ki-67表达呈正相关，胃癌组织中Hp感染可能促进上述蛋白表达；胃癌组织中EBV或Hp感染和EBV、Hp联合感染分别对HER2、P53、Ki-67蛋白表达无增强作用，两者之间无相关性，EBV、Hp可能是胃癌发生发展中的两个独立因素。 Objective: This study is aimed to evaluate the correlation between Epstein-Barr virus, Helicobacter pylori infection and the expression of human epidermal growth factor receptor-2, P53 and Ki-67 in Gastric Cancer tissues, which is helpful to determine the correlation between those two pathogen and gastric cancer. Methods: A total of 78 cases of tissue wax blocks for radical gastric cancer surgery in our Hospital were collected. In situ hybridization was used to detect EBV infection in gastric cancer tissues, and immunohistochemical methods were used to detect the status of HP infection and the expression of HER2, P53, Ki-67 and other proteins in gastric cancer tissues. Results: The positive rate of EBV infection was 12.8% (10/78) and the positive rate of HP was 57.7% (45/78). There is no consistency between the two infections. There were no significant differences between EBV and HP infections in gastric cancer tissues, including gender, age, lymph node metastasis, gastric adenocarcinoma differentiation, depth of invasion, and clinical stage. The expression of HER2, P53, Ki-67 and other proteins in gastric cancer tissues were not significantly different from sex, age, lymph node metastasis, adenocarcinoma differentiation, invasion depth, and clinical stage. The expressions of P53 and Ki-67 proteins were significantly different from Hp infection. There was a positive correlation between the expression of P53 and Ki-67 and Hp infection. There was no significant difference in HER2 protein expression between Hp-positive group and Hp-negative group. There was no significant difference in the expression of P53 and Ki-67 protein between EBV positive or HP positive group and EBV combined HP infection group. Conclusion: There were significant differences in the expression of P53 and Ki-67 in gastric cancer tissues between the HP infection group and the non-infection group, and Hp infection was positively correlated with the expression of P53 and Ki-67. Hp infection in gastric cancer tissues may promote the above protein expression. EBV or HP infection and EBV and Hp infection in gastric cancer tissues have no effect on the enhancement of P53 and Ki-67 protein expression, respectively, and there is no correlation between them. EBV and Hp may be two independent factors in the development of gastric cancer.