Low-Dose Electron Crystallography: Structure Solution and Refinement
Open Access
- 26 January 2022
- Vol. 14 (2), 245
- https://doi.org/10.3390/sym14020245
Abstract
There is a wealth of materials that are beam sensitive and only exist in nanometric crystals, because the growth of bigger crystals is either impossible or so complicated that it is not reasonable to spend enough time and resources to grow big crystals before knowing their potential for research or applications. This difficulty is encountered in minerals, zeolites, metal-organic frameworks or molecular crystals, including pharmaceuticals and biological crystals. In order to study these crystals a structure determination method for beam sensitive crystals of nanometric size is needed. The nanometric size makes them destined for electron diffraction, since electrons interact much more strongly with matter than X-rays or neutrons. In addition, for the same amount of beam damage, electron diffraction yields more information than X-rays. The recently developed low-dose electron diffraction tomography (LD-EDT) not only combines the advantages inherent in electron diffraction, but is also optimized for minimizing the electron dose used for the data collection. The data quality is high, allowing not only the solution of complex unknown structures, but also their refinement taking into account the dynamical diffraction effects. Here we present several examples of crystals solved and refined by this method. The range of the crystals presented includes two synthetic oxides, Sr5CuGe9O24 and (Na2/3Mn1/3)3Ge5O12, a natural mineral (bulachite), and a metal organic framework (Mn-formiate). The dynamical refinement can be successfully performed on data sets that needed less than 0.1 e−/Å2 for the entire data set.Keywords
This publication has 24 references indexed in Scilit:
- The quality of precession electron diffraction data is higher than necessary for structure solution of unknown crystalline phasesActa Crystallographica Section A Foundations of Crystallography, 2011
- “Ab initio” structure solution from electron diffraction data obtained by a combination of automated diffraction tomography and precession techniqueUltramicroscopy, 2009
- Towards automated diffraction tomography. Part II—Cell parameter determinationUltramicroscopy, 2008
- Synthetic aenigmatite analog Na2(Mn5.26Na0.74)Ge6O20: structure and crystal chemical considerationsActa Crystallographica Section C Crystal Structure Communications, 2008
- Synchrotron X‐Ray Charge‐Density Study of Coordination Polymer [Mn(HCOO)2(H2O)2]∞Chemistry – A European Journal, 2007
- Towards automated diffraction tomography: Part I—Data acquisitionUltramicroscopy, 2007
- SUPERFLIP– a computer program for the solution of crystal structures by charge flipping in arbitrary dimensionsJournal of Applied Crystallography, 2007
- The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological moleculesQuarterly Reviews of Biophysics, 1995
- Double conical beam-rocking system for measurement of integrated electron diffraction intensitiesUltramicroscopy, 1994
- Garnet-Like Structures of High-Pressure Cadmium Germanate and Calcium GermanateScience, 1969