Comparison of microbiota of recycled manure solids and straw bedding used in dairy farms in eastern Canada
Open Access
- 1 January 2022
- journal article
- research article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 105 (1), 389-408
- https://doi.org/10.3168/jds.2021-20523
Abstract
Recycled manure solids (RMS) bedding is an alterna-tive bedding option that is growing in popularity on Canadian dairy farms. However, the microbiological characteristics and production of RMS bedding are poorly documented under on-farm conditions in eastern Canada. This bedding could support the presence of pathogens and could have an effect on cow and human health. The aim of this study was to describe the RMS microbiota when used under dairy cows and compare it with straw bedding. Unused and used bedding from 27 RMS and 61 straw-bedded dairy farms were collected and compared using 16S amplicon sequencing, bacterial counts, and Salmonella spp. and Listeria monocytogenes detection. Microbiota composition of unused RMS and unused straw were different. After use, both bedding microbiota were similar in their bacterial composition, structure, and diversity. Unused RMS generally con-tained higher bacterial counts than did unused straw, except for Klebsiella spp. counts. Salmonella spp. and L. monocytogenes were more frequently detected in unused RMS (Salmonella spp.: 11%; L. monocytogenes: 30%), than in unused straw (Salmonella spp.: 0%; L. mono-cytogenes: 11%). Finally, 2 RMS production systems (extraction of the liquid fraction followed by matura-tion in an opened or enclosed container vs. in a heap) did not influence the microbiota richness and bacteria distribution (alpha-diversity), but did influence the micro-biota structure (beta-diversity). In conclusion, animal and human pathogens were found in greater numbers and more frequently in unused RMS than unused straw, and this could eventually affect dairy cow or human health.Keywords
This publication has 36 references indexed in Scilit:
- Short communication: Environmental mastitis pathogen counts in freestalls bedded with composted and fresh recycled manure solidsJournal of Dairy Science, 2016
- Recycling manure as cow bedding: Potential benefits and risks for UK dairy farmsThe Veterinary Journal, 2015
- Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing PlatformApplied and Environmental Microbiology, 2013
- A case of bovine raw milk contamination with Listeria monocytogenesIrish Veterinary Journal, 2012
- Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platformsThe ISME Journal, 2012
- Influence of wet distillers grains diets on beef cattle fecal bacterial community structureBMC Microbiology, 2012
- Bacterial community dynamics in aerated cow manure slurry at different aeration intensitiesJournal of Applied Microbiology, 2011
- Development and validation of a bilingual questionnaire for measuring udder health related management practices on dairy farmsPreventive Veterinary Medicine, 2010
- Evaluation of a multiplex PCR assay as an alternative method for Listeria monocytogenes serotypingJournal of Microbiological Methods, 2010
- Composting of animal manures and chemical criteria for compost maturity assessment. A reviewBioresource Technology, 2009