Durable CRISPR-Based Epigenetic Silencing
Open Access
- 1 January 2021
- journal article
- Published by American Association for the Advancement of Science (AAAS) in BioDesign Research
Abstract
Development of CRISPR-based epigenome editing tools is important for the study and engineering of biological behavior. Here, we describe the design of a reporter system for quantifying the ability of CRISPR epigenome editors to produce a stable gene repression. We characterize the dynamics of durable gene silencing and reactivation, as well as the induced epigenetic changes of this system. We report the creation of single-protein CRISPR constructs bearing combinations of three epigenetic editing domains, termed KAL, that can stably repress the gene expression. This system should allow for the development of novel epigenome editing tools which will be useful in a wide array of biological research and engineering applications.Keywords
Funding Information
- National Institutes of Health Common Fund 4D Nucleome Program (U01 DK127405)
- Li Ka Shing Foundation
- Alfred P. Sloan Foundation
- Pew Charitable Trusts
- National Science Foundation Graduate Research Fellowship Program
- Stanford ChEM-H Undergraduate Research Fellow and Bio-X REU programs
- Stanford School of Medicine
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