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Adult Mouse Spleen Dissociation (On ice) v2
Home
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Adult Mouse Spleen Dissociation (On ice) v2
Adult Mouse Spleen Dissociation (On ice) v2
AP
Andrew Potter
Andrew Potter
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19 June 2018
preprint
Published by
ZappyLab, Inc.
https://doi.org/10.17504/protocols.io.q5cdy2w
Abstract
Protocol used to dissociate adult (8-10 wk) mouse spleen into single cells. Attained >95% viability, a variety of cell sizes, and ~10 million cells from 12 mg tissue.
Keywords
ADULT
VIABILITY
DISSOCIATE
ICE
TISSUE
MOUSE SPLEEN DISSOCIATION