Identification and Characterization Roles of Phytoene Synthase (PSY) Genes in Watermelon Development
Open Access
- 1 July 2022
- Vol. 13 (7), 1189
- https://doi.org/10.3390/genes13071189
Abstract
Phytoene synthase (PSY) plays an essential role in carotenoid biosynthesis. In this study, three ClPSY genes were identified through the watermelon genome, and their full-length cDNA sequences were cloned. The deduced proteins of the three ClPSY genes were ranged from 355 to 421 amino acid residues. Phylogenetic analysis suggested that the ClPSYs are highly conserved with bottle gourd compared to other cucurbit crops PSY proteins. Variation in ClPSY1 expression in watermelon with different flesh colors was observed; ClPSY1 was most highly expressed in fruit flesh and associated with the flesh color formation. ClPSY1 expression was much lower in the white-fleshed variety than the colored fruits. Gene expression analysis of ClPSY genes in root, stem, leaf, flower, ovary and flesh of watermelon plants showed that the levels of ClPSY2 transcripts found in leaves was higher than other tissues; ClPSY3 was dominantly expressed in roots. Functional complementation assays of the three ClPSY genes suggested that all of them could encode functional enzymes to synthesize the phytoene from Geranylgeranyl Pyrophosphate (GGPP). Some of the homologous genes clustered together in the phylogenetic tree and located in the synteny chromosome region seemed to have similar expression profiles among different cucurbit crops. The findings provide a foundation for watermelon flesh color breeding with regard to carotenoid synthesis and also provide an insight for the further research of watermelon flesh color formation.Funding Information
- National Natural Science Foundation of China (32172577)
This publication has 48 references indexed in Scilit:
- Dissection of Tomato Lycopene Biosynthesis through Virus-Induced Gene SilencingPlant Physiology, 2013
- The genome of melon ( Cucumis melo L.)Proceedings of the National Academy of Sciences of the United States of America, 2012
- The Tomato MADS-Box Transcription Factor RIPENING INHIBITOR Interacts with Promoters Involved in Numerous Ripening Processes in a COLORLESS NONRIPENING-Dependent MannerPlant Physiology, 2011
- CDD: a Conserved Domain Database for the functional annotation of proteinsNucleic Acids Research, 2010
- Provitamin A Accumulation in Cassava (Manihot esculenta) Roots Driven by a Single Nucleotide Polymorphism in a Phytoene Synthase GeneTHE PLANT CELL ONLINE, 2010
- Allotopic expression of mitochondrial-encoded genes in mammals: achieved goal, undemonstrated mechanism or impossible task?Nucleic Acids Research, 2010
- Cucumber ( Cucumis sativus ) and melon ( C. melo ) have numerous wild relatives in Asia and Australia, and the sister species of melon is from AustraliaProceedings of the National Academy of Sciences of the United States of America, 2010
- Source to sink: regulation of carotenoid biosynthesis in plantsTrends in Plant Science, 2010
- trimAl: a tool for automated alignment trimming in large-scale phylogenetic analysesBioinformatics, 2009
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001