Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway
Open Access
- 1 January 2019
- journal article
- research article
- Published by The Company of Biologists in Biology Open
- Vol. 8 (7)
- https://doi.org/10.1242/bio.043653
Abstract
The aim of this study was to investigate whether exogenous hydrogen sulfide (H2S) could mitigate NLRP3 inflammasome-mediated inflammation through promoting autophagy via the AMPK-mTOR pathway in L02 cells. L02 cells were stimulated with different concentrations of oleic acid (OA), then cell viability and the protein expression of NLRP3 and pro-caspase-1 were detected by MTT and western blot, respectively, to determine appropriate OA concentration in this study. The cells were divided into four groups: the cells in the control group were cultured with RPMI-1640 for 24.5 h; the cells in the OA group were cultured with RPMI-1640 for 0.5 h, then were stimulated with 1.2 mmol/l OA for 24 h; the cells in the NaHS+OA group were pretreated with sodium hydrogen sulfide (NaHS, a donor of H2S) for 0.5 h before exposure to OA for 24 h; and the cells in the NaHS group were treated with NaHS 0.5 h, then were cultured with RPMI-1640 for 24 h. Subsequently, the cells in every group were collected and the protein expression of NLRP3, procaspase-1, cleaved caspase-1, P62, LC3, Beclin1, T-AMPK, P-AMPK, T-mTOR, P-mTOR and the level of IL-1β were detected by western blot and ElISA, respectively. Exogenous H2S reduced the level of NLRP3, caspase-1, P62, IL-1β and the ratio of P-mTOR/T-mTOR induced by OA and increased the ratio of LC3 II/I and the protein expression of Beclin1 suppressed by OA. This study demonstrates for the first time that H2S might suppress NLRP3 inflammasome-mediated inflammation induced by OA through promoting autophagy via the AMPK-mTOR pathway. It provides a theoretical basis for the further study of the anti-inflammatory mechanism of H2S.Funding Information
- Key Scientific Research Project of Colleges and Universities in Henan Province (16A310001)
This publication has 42 references indexed in Scilit:
- An Overview of Autophagy: Morphology, Mechanism, and RegulationAntioxidants and Redox Signaling, 2014
- Hydrogen sulfide protected gastric epithelial cell from ischemia/reperfusion injury by Keap1 s-sulfhydration, MAPK dependent anti-apoptosis and NF-κB dependent anti-inflammation pathwayEuropean Journal of Pharmacology, 2014
- Autophagy as a Stress-Response and Quality-Control Mechanism: Implications for Cell Injury and Human DiseaseAnnual review of pathology, 2013
- A Double‐Edged Sword with Therapeutic Potential: An Updated Role of Autophagy in Ischemic Cerebral InjuryCNS Neuroscience & Therapeutics, 2012
- Autophagy modulation as a potential therapeutic target for diverse diseasesNature Reviews Drug Discovery, 2012
- The early autophagic pathway is activated by hepatitis B virus and required for viral DNA replicationProceedings of the National Academy of Sciences of the United States of America, 2010
- Hydrogen sulfide therapy attenuates the inflammatory response in a porcine model of myocardial ischemia/reperfusion injuryThe Journal of Thoracic and Cardiovascular Surgery, 2009
- Hydrogen sulfide attenuates myocardial ischemia-reperfusion injury by preservation of mitochondrial functionProceedings of the National Academy of Sciences of the United States of America, 2007
- Distinct Roles of Autophagy in the Heart During Ischemia and ReperfusionCirculation Research, 2007
- The InflammasomeMolecular Cell, 2002