Anticancer and Antioxidant Activity of the Greenly Synthesized Zinc Nanoparticles Composites using Aqueous Extract of Withania Somnifera plant
- 30 May 2021
- journal article
- research article
- Published by Egypts Presidential Specialized Council for Education and Scientific Research in Egyptian Journal of Chemistry
- Vol. 64 (10), 5561-5574
- https://doi.org/10.21608/ejchem.2021.75114.3686
Abstract
Withania somnifera L. is a medicinal herb related to Solanaceae family, known as ashwagandha. The aqueous extract of W. somnifera was utilized for the green synthesis of zinc nanoparticles to investigated their antioxidant and anticancer potentials. The synthesized zinc nanoparticle solution of W. somnifera extract was characterized by phytochemical analysis, Transmission Electron Microscope (TEM), and zeta potential. The results revealed reduced values of the phytochemical constitutes, large surface area, and high stability of the nanoparticles. The antioxidant activity of the extracted W. somnifera and its zinc nanoparticles was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH•) assay, in which the original extract has the more antioxidant capacity with IC50= 0.701 mg/mL, along with potent results for both samples relative to ascorbic acid. The extracted W. somnifera, its zinc nanoparticles, and zinc sulfate solution were in vitro assessed as anticancer agents on well-known six human tumor, and a normal cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The results demonstrated that the synthesized zinc nanoparticles of the W. somnifera extract shown the most potent cytotoxicity with IC50= 19.17 µg/mL on HeLa cell line. The synthesized nano-zinc solution of the W. somnifera aqueous extract performed to be proficient as a potent anticancer characteristic than the plant extract with developed biological potency.Keywords
This publication has 42 references indexed in Scilit:
- Water Extract of Ashwagandha Leaves Has Anticancer Activity: Identification of an Active Component and Its Mechanism of ActionPLOS ONE, 2013
- A Prospective, Randomized Double-Blind, Placebo-Controlled Study of Safety and Efficacy of a High-Concentration Full-Spectrum Extract of Ashwagandha Root in Reducing Stress and Anxiety in AdultsIndian Journal of Psychological Medicine, 2012
- Synthesis and antitumor evaluation of some new 1,3,4-oxadiazole-based heterocyclesEuropean Journal of Medicinal Chemistry, 2012
- Synthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agentsInternational Journal of Nanomedicine, 2012
- Withaferin A targets heat shock protein 90 in pancreatic cancer cellsBiochemical Pharmacology, 2010
- In Vitro anticancer activity of the root, stem and leaves of Withania Somnifera against various human cancer cell linesIndian Journal of Pharmaceutical Sciences, 2010
- Effect of the alcoholic extract of Ashwagandha leaves and its components on proliferation, migration, and differentiation of glioblastoma cells: Combinational approach for enhanced differentiationCancer Science, 2009
- Withanolide sulfoxide from Aswagandha roots inhibits nuclear transcription factor‐kappa‐B, cyclooxygenase and tumor cell proliferationPhytotherapy Research, 2009
- Reactive oxygen species generation and mitochondrial dysfunction in the apoptotic cell death of human myeloid leukemia HL-60 cells by a dietary compound withaferin A with concomitant protection by N-acetyl cysteineApoptosis, 2007
- Antioxidant Activity of Apple PeelsJournal of Agricultural and Food Chemistry, 2003