Genome-Wide Identification and Structural Analysis of bZIP Transcription Factor Genes in Brassica napus
Open Access
- 24 October 2017
- Vol. 8 (10), 288
- https://doi.org/10.3390/genes8100288
Abstract
The basic region/leucine zipper motif (bZIP) transcription factor family is one of the largest families of transcriptional regulators in plants. bZIP genes have been systematically characterized in some plants, but not in rapeseed (Brassica napus). In this study, we identified 247 BnbZIP genes in the rapeseed genome, which we classified into 10 subfamilies based on phylogenetic analysis of their deduced protein sequences. The BnbZIP genes were grouped into functional clades with Arabidopsis genes with similar putative functions, indicating functional conservation. Genome mapping analysis revealed that the BnbZIPs are distributed unevenly across all 19 chromosomes, and that some of these genes arose through whole-genome duplication and dispersed duplication events. All expression profiles of 247 bZIP genes were extracted from RNA-sequencing data obtained from 17 different B. napus ZS11 tissues with 42 various developmental stages. These genes exhibited different expression patterns in various tissues, revealing that these genes are differentially regulated. Our results provide a valuable foundation for functional dissection of the different BnbZIP homologs in B. napus and its parental lines and for molecular breeding studies of bZIP genes in B. napus.Keywords
This publication has 78 references indexed in Scilit:
- Genome-Wide Analysis of bZIP-Encoding Genes in MaizeDNA Research, 2012
- Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and CufflinksNature Protocols, 2012
- The ABRE-binding bZIP transcription factor OsABF2 is a positive regulator of abiotic stress and ABA signaling in riceJournal of Plant Physiology, 2010
- Arabidopsis thaliana transcription factors bZIP19 and bZIP23 regulate the adaptation to zinc deficiencyProceedings of the National Academy of Sciences of the United States of America, 2010
- MEME SUITE: tools for motif discovery and searchingNucleic Acids Research, 2009
- Expression patterns within the Arabidopsis C/S1 bZIP transcription factor network: availability of heterodimerization partners controls gene expression during stress response and developmentPlant Molecular Biology, 2008
- Clustal W and Clustal X version 2.0Bioinformatics, 2007
- A central integrator of transcription networks in plant stress and energy signallingNature, 2007
- Combinatorial control of Arabidopsis proline dehydrogenase transcription by specific heterodimerisation of bZIP transcription factorsThe EMBO Journal, 2006
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997