SOLUBILITY OF OXALATE AND ITS ASSOCIATION WITH METAL IONS ASSESSED BY A SIMULATED IN VITRO DIGESTION MODEL
- 1 January 2019
- journal article
- research article
- Published by Pakistan Journal of Agricultural Sciences in Pakistan Journal of Agricultural Sciences
- Vol. 56 (4), 921-927
- https://doi.org/10.21162/pakjas/19.6378
Abstract
Bioavailability of oxalate is highly influenced by the presence of metal cations in the gastrointestinal tract as a result of the different solubility of oxalate salts. Bioavailability is further influenced by pH effects on oxalate solubility at various stages of digestion, particularly at gastric pH at which insoluble oxalate has been found to solubilize. The aim of the present study was to assess the effect of gastrointestinal pH and different metal ions on the relative solubility of oxalate after treatment with relevant gastric and intestinal enzymes in simulated gastrointestinal digestion conditions. Whole bran cereals and beans were used as test samples. Oxalate solubility increased along with reproducible recovery such as 97 +/- 4 at gastric pH-2 compared to higher pH. High amount of soluble oxalate (32 +/- 1.2) was found in wheat bran after simulated in vitro digestion under gastric conditions as compared to intestinal conditions i.e. 11 +/- 0.5. The availability of calcium for absorption was found to be very low compared to that of magnesium and potassium i.e., 15 +/- 0.3, 13 +/- 0.5 to 10 +/- 0.42 and 4 +/- 1 in wheat bran sample at gastric and intestinal conditions simultaneously. The formation of a complex between calcium and oxalate reduced solubility and hence made calcium less available for absorption. Presence of metals and oxalate not only reduces oxalate availability, but it also reduces availability of metal cations.Keywords
This publication has 12 references indexed in Scilit:
- Enzymatic hydrolysis of phytate and effects on soluble oxalate concentration in foodsFood Chemistry, 2017
- Effects of phytate and minerals on the bioavailability of oxalate from foodFood Chemistry, 2013
- Comparison of the estimates of the oxalate content of taro leaves and corms and a selection of Indian vegetables following hot water, hot acid and in vitro extraction methodsJournal of Food Composition and Analysis, 2010
- Oxalate Content of Silver Beet Leaves (Beta vulgaris var. cicla) at Different Stages of Maturation and the Effect of Cooking with Different Milk SourcesJournal of Agricultural and Food Chemistry, 2009
- Oxalates in some Indian green leafy vegetablesInternational Journal of Food Sciences and Nutrition, 2008
- Determination of oxalates in Japanese taro corms using an in vitro digestion assayFood Chemistry, 2007
- Soluble and insoluble oxalate content of nutsJournal of Food Composition and Analysis, 2007
- CALCIUM OXALATE IN PLANTS: Formation and FunctionAnnual Review of Plant Biology, 2005
- Applicability of an in vitro digestion model in assessing the bioaccessibility of mycotoxins from foodFood and Chemical Toxicology, 2005
- Comparison of Five In Vitro Digestion Models To Study the Bioaccessibility of Soil ContaminantsEnvironmental Science & Technology, 2002