THE USE OF GADOLINIUM ORTHOVANADATE NANOPARTICLES FOR THE CORRECTION OF REPRODUCTIVE ABILITY IN BOARS UNDER OXIDATIVE STRESS

Abstract

The search for effective and safe means of correcting male hypofertility is an urgent problem of modern reproductology. Researchers have proved the possibility of the use of nanoparticles based on oxides of rare earth elements for the treatment of disorders of male reproductive function. We investigated the effectiveness of the use of gadolinium orthovanadate nanoparticles activated by europium the size of which was 25×8 nm in the dose of 0,0125 mg per kg of live weight to correct reproductive ability decrease in boars under oxidative stress. After 14 days of hydrosol nanoparticles administration we established experimentally the dynamics of the content markers of oxidative stress and stable metabolites in the Nitrogen oxide cycle, and also determined the changes in sperm quality indicators. Thus, diene conjugates concentration in the blood serum of boars on the 15th day tended to decrease, and on the 30th day of the study was lower by 9,4 % compared to the group of animals before administration. At the same time the amount of thiobarbiturate-active products decreased on the 15th day of the study by 24,7 %, and on the 30th day – by 48,2 %, which indicates the normalization of oxidative processes in males. We noted positive changes in the system of the Nitrogen oxide cycle, the content of stable metabolites on the 15th day was lower than in animals before administration by 25,2 %, and on the 30th day – by 42,6 %. At the same time, we observed an improvement in the quality of boar sperm, especially motility and the number of motile sperm in the ejaculate increased by 42,9 % and 57,1 % on the 60th day and by 95,2 % and 1,48 times on the 90th the day of the study, respectively. In this case, the volume of ejaculate, sperm concentration and sperm content with morphological anomalies with the introduction of nanoparticles normalized and almost reached the values of the control group. The researchers are interested in further elucidating the effect of correction of reduced reproductive ability in boars under oxidative stress with gadolinium orthovanadate nanoparticles on hormonal background and the state of enzymatic and non-enzymatic systems of antioxidant protection.Keywords: sperm quality, prooxidant-antioxidant system, peroxidation, oxidative stress markers, the Nitrogen oxide cycle, nanomaterials