Identification, Screening, and Molecular Characterization of Bacterial Microbiota in the guts of Epinephelus sp.
Open Access
- 31 December 2020
- journal article
- Published by Abasyn University in Abasyn Journal of Life Sciences
Abstract
Bacterial microbiota is predominantly present in all living organisms. Most of the bacteria present in the gut of the fish are contaminating the food chain. In the present study, we aimed to isolate and characterize the bacteria in the gut of Epinephelus sp. in the red sea of Jeddah, Kingdom of Saudi Arabia. Bacteria were isolated from the guts of 10 fish samples and were grown on Luria Bertani (LB) and nutrient agar media. Total thirteen bacterial colonies were screen out by morphological identification i.e., color, shape, structure, etc. which were further reduced to 7 colonies e.g., IF001, IF002, F003, IF004, F005, IF006, and IF007. The bacterial isolates were also identified through molecular identification using 16S-rDNA sequencing. The genomic DNA was isolated and was sequenced using the Sanger® sequencing method. BLAST alignment results that IF001 and IF002 were members Bacillus sp. IF003 was a strain of photobacterium damselae, IF004 and IF006 were strains of Rothia endophytica, IF005 was a strain of Acinetobacter bouvetiiand IF007 was belonged to Shewanella oneidensis. The molecular identification confirmed the identification of bacterial isolates in the Epinephelus sp. obtained from the red sea.Keywords
This publication has 16 references indexed in Scilit:
- Endophytic bacteria isolated from Solanum nigrum L., alleviate cadmium (Cd) stress response by their antioxidant potentials, including SOD synthesis by sodA geneEcotoxicology and Environmental Safety, 2019
- Improvement in phytoremediation potential of Solanum nigrum under cadmium contamination through endophytic-assisted Serratia sp. RSC-14 inoculationEnvironmental Science and Pollution Research, 2015
- Ribosomal Database Project: data and tools for high throughput rRNA analysisNucleic Acids Research, 2013
- Selection of candidate probionts by two different screening strategies from Atlantic cod (Gadus morhua L.) larvaeVeterinary Microbiology, 2010
- The current status and future focus of probiotic and prebiotic applications for salmonidsAquaculture, 2010
- Effect of probiotics on the survival, growth and challenge infection in Tilapia nilotica (Oreochromis niloticus)Aquaculture Research, 2008
- Long-term storage conditions for carriers with denitrifying biomass of the fluidized, methanol-fed denitrification reactor of the Montreal Biodome, and the impact on denitrifying activity and bacterial populationWater Research, 2006
- Statistical analysis of denaturing gel electrophoresis (DGE) fingerprinting patternsEnvironmental Microbiology, 2002
- Basic local alignment search toolJournal of Molecular Biology, 1990
- Confidence Limits on Phylogenies: An Approach Using the BootstrapEvolution, 1985