Antioxidant activity and production of secondary metabolites of adult plant and in vitro calli of Anodendron paniculatum

Abstract

The members of the family Apocynaceae are the rich in alkaloids, terpinoids, flavonoids, phenolic compounds and hydrocarbons. Anodendron paniculatum Roxb. is an woody climber, the roots of which have been used to control vomiting and cough. In the present study, adult plant and tissue culture plant extracts of A. paniculatum collected from Eastern Ghats, Araku, Andhra Pradesh were used for the production of their secondary metabolites and antioxidant activity. Methanol extracts of calli showed high contents of total phenol and alkaloid with 25.53 and 25.98 mg/g respectively. Methanol extract of tissue culture plant at higher concentrations showed better antioxidant activity with reference to standard ascorbic acid. For this study different concentrations (12.5, 25, 50, 100 and 200 µg/ml) of extracts were prepared by using four different solvents. Tissue culture extracts from methanol showed maximum scavenging activity with 231.9±1.39 % at 200 µg/ml of concentration, while that of adult plant extracts showed the scavenging activity of 189.1±0.74% only. These results on high antioxidant activity of the methanol extract of A. paniculatum calli can be attributed to the presence of high phenolic and alkaloid contents in calli when compared to that of adult plant extract.