Assessment of multiple biomarkers in Lithobates catesbeianus (Anura: Ranidae) tadpoles exposed to zinc oxide nanoparticles and zinc chloride: integrating morphological and behavioral approaches to ecotoxicology
- 23 September 2022
- journal article
- research article
- Published by Springer Science and Business Media LLC in Environmental Science and Pollution Research
- Vol. 30 (5), 13755-13772
- https://doi.org/10.1007/s11356-022-23018-4
Abstract
The ecotoxicological risk to vertebrates posed by zinc oxide nanoparticles (ZnO NPs) is still poorly understood, especially in animals with a biphasic life cycle, which have aquatic and terrestrial phases, such as amphibians. In the present study, we investigated whether acute exposure (7 days) to ZnO NPs and zinc chloride (ZnCl2) at three environmentally relevant concentrations (0.1, 1.0, and 10 mg L−1) induces changes in the morphology, chondrocranium, and behavior of the tadpoles of Lithobates catesbeianus (Anura: Ranidae). Tadpoles exposed to both forms of Zn did not undergo any morphological or behavioral changes at the lowest concentrations (0.1 and 1.0 mg L−1). However, the animals exposed to the highest concentration (10 mg L−1) lacked oral disc structures, were smaller in size, had a longer tail, and presented changes in the position and coiling of the intestine and malformations of the chondrocranium in comparison with the control group. This indicates that ZnO NPs and ZnCl2 altered the development of the tadpoles, causing delays in their metamorphosis and even reducing individual fitness. The tadpoles exposed to both forms of Zn at 10 mg L−1 also had reduced mobility, especially in the presence of conspecifics. Based on these findings, we emphasize the importance of studying morphological, skeletal, and behavioral biomarkers to evaluate the toxic effects of metal-based nanoparticles in amphibians. Graphical abstractFunding Information
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (88887.374100/2019–00)
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (20181002600, 307652/2018-1, 306329/2020-4)
- Fundação de Amparo à Pesquisa do Estado de Goiás (201810267001786)
This publication has 110 references indexed in Scilit:
- Making Friends: Social Attraction in Larval Green and Golden Bell Frogs, Litoria aureaPLOS ONE, 2013
- Polyaniline nanofibers: Acute toxicity and teratogenic effect on Rhinella arenarum embryosChemosphere, 2012
- Effect of titanium dioxide nanomaterials and ultraviolet light coexposure on African clawed frogs (Xenopus laevis)Environmental Toxicology and Chemistry, 2011
- Nanometals Induce Stress and Alter Thyroid Hormone Action in Amphibia at or below North American Water Quality GuidelinesEnvironmental Science & Technology, 2010
- The Impact of Variation in Labial Tooth Number on the Feeding Kinematics of Tadpoles of Southern Leopard Frog (Lithobates sphenocephalus)Ichthyology & Herpetology, 2010
- The cause of global amphibian declines: a developmental endocrinologist's perspectiveJournal of Experimental Biology, 2010
- Generalized Linear Mixed ModelsPublished by Wiley ,2005
- Behavioural responses to predator chemical cues and local variation in antipredator performance in Rana temporaria tadpolesOikos, 2000
- Sequence of ontogenetic development and atrophy of the oral apparatus of six anuran tadpolesJournal of Morphology, 1988
- Surface anatomy of branchial food traps of tadpoles: A comparative studyJournal of Morphology, 1979