Fatty acid–binding protein genes in gilthead seabream: molecular cloning and nutritional regulation under low water temperatures
- 3 February 2023
- journal article
- research article
- Published by Wiley in Journal of Fish Biology
- Vol. 102 (4), 816-828
- https://doi.org/10.1111/jfb.15319
Abstract
The molecular characteristics and tissue disruption of 10 fatty acid-binding protein (fabp) genes in gilthead seabream (Sparus aurata) were investigated, and their expression levels were found in the fish fed diets with different vegetable oil (VO) sources, which may explore the potential function of fabp genes in S. aurata. For this purpose, the open reading frames of fabp genes involved in the transport and beta-oxidation of fatty acids (FA) were molecularly cloned and characterized. S. aurata was then exposed to a two-staged feeding trial (the grow-out period following a wash-out period) at low water temperatures. In the grow-out period, the fish were fed diets containing 50% and 100% ratios of various VOs for 60 days, and in the wash-out period, the fish were fed a diet containing 100% fish oil (FO) for 30 days. It has been determined that (a) S. aurata and vertebrate fabp/FABP genes are orthologues; (b) spatio-temporal differences in tissue-specific patterns of fabp genes differ importantly; for instance, the difference between the highest and lowest values reaches 13 x 10(5)-fold in the fabp10a; and (c) VO-based diets upregulated fabp transcript levels in the liver and muscle with some exceptions, such as liver fabp11a and muscle fabp7a. Gene expressions of only the hepatic fabp7b and fabp10a genes were diminished at the end of the wash-out period. In this study, the authors provide further evidence that dietary FAs affect fabp mRNA expressions in S. aurata. This might be useful in the nutritional control of fabp genes to maintain lipid homeostasis in marine fish fed VO-based diets at low water temperatures.Keywords
Funding Information
- Çukurova Üniversitesi (FDK‐2017‐9418)
This publication has 59 references indexed in Scilit:
- Pathological Investigations of Winter-like Disease in Cultured Sea Breams (Sparus aurata)Kafkas Universitesi Veteriner Fakultesi Dergisi, 2013
- Tissue-specific Functions in the Fatty Acid-binding Protein FamilyOnline Journal of Public Health Informatics, 2010
- Differential transcriptional modulation of duplicated fatty acid-binding protein genes by dietary fatty acids in zebrafish (Danio rerio): evidence for subfunctionalization or neofunctionalization of duplicated genesBMC Evolutionary Biology, 2009
- trimAl: a tool for automated alignment trimming in large-scale phylogenetic analysesBioinformatics, 2009
- Hierarchical subfunctionalization of fabp1a, fabp1b and fabp10 tissue‐specific expression may account for retention of these duplicated genes in the zebrafish (Danio rerio) genomeThe FEBS Journal, 2006
- From 2R to 3R: evidence for a fish‐specific genome duplication (FSGD)BioEssays, 2005
- The Cellular Retinol-Binding Protein Genes Are Duplicated and Differentially Transcribed in the Developing and Adult Zebrafish (Danio rerio)Molecular Biology and Evolution, 2004
- Spatio‐temporal distribution of cellular retinol‐binding protein gene transcripts (CRBPI and CRBPII) in the developing and adult zebrafish (Danio rerio)JBIC Journal of Biological Inorganic Chemistry, 2003
- Functional alterations associated with “winter syndrome” in gilthead sea bream (Sparus aurata)Aquaculture, 2003
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001